LOCUS       BBa_S03554              2477 bp    DNA     linear       22-Nov-2024
DEFINITION  S0104:S03545
ACCESSION   BBa_S03554
VERSION     BBa_S03554.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     misc_feature    1..712
                     /label=BBa_S0104
     misc_feature    725..728
                     /label=conserved
     RBS             721..732
                     /label=BBa_B0034
     misc_feature    739..741
                     /label=start
     misc_feature    1522..1527
                     /label=2
     misc_feature    1489..1521
                     /label=LVA
     CDS             739..1488
                     /label=aiiA
     misc_feature    739..1527
                     /label=BBa_C0060
     misc_feature    1528..1552
                     /label=Help:Barcodes
     CDS             739..1527
                     /label=BBa_C0060
     misc_feature    721..1552
                     /label=BBa_C0260
     misc_feature    1565..1568
                     /label=conserved
     RBS             1561..1572
                     /label=BBa_B0034
     CDS             1579..2340
                     /label=YFP (LVA)
     misc_feature    2335..2340
                     /label=2
     misc_feature    2296..2334
                     /label=SsrA
     misc_feature    1820..1820
                     /label=A (Q->L)
     misc_feature    1579..2340
                     /label=BBa_E0032
     misc_feature    1579..2340
                     /label=BBa_E0032
     stem_loop       2360..2403
                     /label=stem_loop
     misc_feature    2349..2428
                     /label=BBa_B0010
     terminator      2349..2428
                     /label=BBa_B0010
     stem_loop       2444..2463
                     /label=T7 TE
     misc_feature    2470..2470
                     /label=stop
     polyA_site      2464..2477
                     /label=polya
     misc_feature    2437..2477
                     /label=BBa_B0012
     terminator      2437..2477
                     /label=BBa_B0012
     misc_feature    1561..2477
                     /label=BBa_E0432
     misc_feature    721..2477
                     /label=BBa_S03545
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt
      781 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg
      841 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca
      901 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta
      961 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg
     1021 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc
     1081 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc
     1141 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat
     1201 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa
     1261 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat
     1321 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta
     1381 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga
     1441 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac
     1501 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1561 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1741 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
     1801 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2041 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2161 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2281 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
     2341 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2401 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2461 gcctttctgc gtttata
//