##gff-version 3
##sequence-region BBa_S03558 1 2327
BBa_S03558	.	engineered_region	1	1405	.	+	0	ID=BBa_S03542;Name=BBa_S03542
BBa_S03558	.	engineered_region	721	1405	.	+	0	ID=BBa_I9026;Name=BBa_I9026;Parent=BBa_S03542
BBa_S03558	.	ribosome_entry_site	721	732	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I9026
BBa_S03558	.	conserved_region	725	728	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03558	.	CDS	739	1380	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I9026
BBa_S03558	.	tag	1342	1374	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_S03558	.	engineered_tag	1381	1405	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_S03558	.	CDS	739	1341	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_S03558	.	engineered_region	1	712	.	+	0	ID=BBa_S0104;Name=BBa_S0104;Parent=BBa_S03542
BBa_S03558	.	CDS	19	687	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_S0104
BBa_S03558	.	engineered_tag	688	712	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_S03558	.	tag	649	687	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_S03558	.	engineered_region	19	687	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_S03558	.	CDS	19	687	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_S03558	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0104
BBa_S03558	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03558	.	engineered_region	1414	2327	.	+	0	ID=BBa_S03553;Name=BBa_S03553
BBa_S03558	.	ribosome_entry_site	1414	1425	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03553
BBa_S03558	.	conserved_region	1418	1421	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03558	.	engineered_region	1432	2190	.	+	0	ID=BBa_E0044;Name=BBa_E0044;Parent=BBa_S03553
BBa_S03558	.	terminator	2199	2278	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03553
BBa_S03558	.	stem_loop	2210	2253	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03558	.	engineered_region	2199	2278	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03558	.	terminator	2287	2327	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03553
BBa_S03558	.	engineered_region	2287	2327	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03558	.	stop_codon	2320	2320	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03558	.	polyA_site	2314	2327	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03558	.	stem_loop	2294	2313	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_S03558
aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaac
ttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttacc
agaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttggg
cacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacg
atatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtga
ttccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaat
ggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagt
acctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagc
gagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcg
ctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtga
ccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgct
cgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacc
cgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctc
cctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgt
aacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggctt
accaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggag
aaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaaaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata