##gff-version 3
##sequence-region BBa_S03565 1 2826
BBa_S03565	.	CDS	1414	2604	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03565	.	primer_binding_site	2585	2604	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03565	.	primer_binding_site	1414	1437	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03565	.	CDS	1414	2604	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03565	.	stop_codon	2602	2604	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03565	.	ribosome_entry_site	1393	1407	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03565	.	ribosome_entry_site	1400	1404	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03565	.	engineered_region	1393	1407	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03565	.	ribosome_entry_site	1393	1407	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03565	.	engineered_region	1	1384	.	+	0	ID=BBa_S03563;Name=BBa_S03563
BBa_S03565	.	CDS	194	1384	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03563
BBa_S03565	.	primer_binding_site	1365	1384	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03565	.	primer_binding_site	194	217	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03565	.	CDS	194	1384	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03565	.	stop_codon	1382	1384	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03565	.	ribosome_entry_site	173	187	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_S03563
BBa_S03565	.	ribosome_entry_site	180	184	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03565	.	engineered_region	173	187	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03565	.	ribosome_entry_site	173	187	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03565	.	promoter	1	130	.	+	0	ID=BBa_J44002;Name=BBa_J44002;Parent=BBa_S03563
BBa_S03565	.	promoter	1	130	.	+	0	ID=annotation2002776;Name=promoter;Parent=BBa_J44002
BBa_S03565	.	sequence_feature	139	164	.	+	0	ID=BBa_J44000;Name=BBa_J44000;Parent=BBa_S03563
BBa_S03565	.	sequence_feature	2750	2826	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03565	.	sequence_feature	2800	2800	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03565	.	non_covalent_binding_site	2804	2818	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03565	.	sequence_feature	2826	2826	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03565	.	sequence_feature	2778	2778	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03565	.	non_covalent_binding_site	2755	2770	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03565	.	sequence_feature	2750	2826	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03565	.	sequence_feature	2800	2805	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03565	.	terminator	2613	2741	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S03565	.	terminator	2613	2692	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03565	.	engineered_region	2613	2692	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03565	.	stem_loop	2624	2667	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03565	.	terminator	2701	2741	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03565	.	stop_codon	2734	2734	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03565	.	polyA_site	2728	2741	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03565	.	stem_loop	2708	2727	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03565	.	engineered_region	2701	2741	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S03565
gctagcccaaaaaaacggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatgg
ataaaaatgctatggcatagcaaagtgtgacgccgtgcaaataatcaatgttactagagttatcaaaaaccatggtttt
tgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcac
cctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatc
gccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccg
accgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacc
cgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatc
gccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcag
gccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaa
cctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaaccca
gtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtag
gacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgct
tgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcag
gccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttcc
ccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacga
ccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcg
atttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccg
cgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagattaaagaggagaaatactagatgaaatct
aacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgg
gcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgca
atttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttgga
gccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatca
ccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggct
catgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgca
ccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggag
agcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgc
acttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgc
tttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtca
ctggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtctt
gctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcg
ttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcc
taacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggat
tgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcgggtgtcaacaat
tgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg