LOCUS       BBa_S03566              1606 bp    DNA     linear       22-Dec-2024
DEFINITION  pBADrev-hixC-RBS-TetF-TT-RE
ACCESSION   BBa_S03566
VERSION     BBa_S03566.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=promoter
     promoter        1..130
                     /label=BBa_J44002
     misc_feature    139..164
                     /label=BBa_J44000
     RBS             173..187
                     /label=RBS-1\Strong
     RBS             180..184
                     /label=RBS
     misc_feature    173..187
                     /label=BBa_B0030
     RBS             173..187
                     /label=BBa_B0030
     CDS             194..1384
                     /label=tetA(C) forward
     primer_bind     194..217
                     /label=Fwd primer J31007
     primer_bind     1365..1384
                     /label=Rev primer J31007
     misc_feature    1382..1384
                     /label=stop
     CDS             194..1384
                     /label=BBa_J31007
     stem_loop       1404..1447
                     /label=stem_loop
     misc_feature    1393..1472
                     /label=BBa_B0010
     terminator      1393..1472
                     /label=BBa_B0010
     stem_loop       1488..1507
                     /label=T7 TE
     misc_feature    1514..1514
                     /label=stop
     polyA_site      1508..1521
                     /label=polya
     misc_feature    1481..1521
                     /label=BBa_B0012
     terminator      1481..1521
                     /label=BBa_B0012
     misc_feature    1580..1585
                     /label=Former SpeI site
     misc_feature    1580..1580
                     /label=Mutation of SpeI site
     misc_feature    1530..1606
                     /label=RE Sequence
     misc_feature    1558..1558
                     /label=Originally a C
     misc_feature    1606..1606
                     /label=Insertion right before biobrick ends
     misc_feature    1535..1550
                     /label=Proximal Fis Binding Site
     misc_feature    1584..1598
                     /label=Distal Fis Binding Site
     misc_feature    1530..1606
                     /label=BBa_J3101
ORIGIN
        1 gctagcccaa aaaaacggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta
       61 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
      121 taatcaatgt tactagagtt atcaaaaacc atggtttttg ataatactag agattaaaga
      181 ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
      241 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
      301 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
      361 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
      421 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
      481 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      541 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      601 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      661 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      721 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
      781 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
      841 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
      901 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
      961 aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac gtttcggcga
     1021 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     1081 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     1141 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     1201 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
     1261 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     1321 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     1381 ctaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1441 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1501 gtgggccttt ctgcgtttat atactagagt tcgggtgtca acaattgacc aaaatattga
     1561 tttacagcgt aatgcgcttt ctagtgcaaa ttgtgaccgc attttg
//