##gff-version 3
##sequence-region BBa_S03572 1 1434
BBa_S03572	.	sequence_feature	1358	1434	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03572	.	non_covalent_binding_site	1412	1426	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03572	.	sequence_feature	1408	1408	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03572	.	sequence_feature	1434	1434	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03572	.	sequence_feature	1386	1386	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03572	.	non_covalent_binding_site	1363	1378	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03572	.	sequence_feature	1358	1434	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03572	.	sequence_feature	1408	1413	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03572	.	terminator	1309	1349	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03572	.	polyA_site	1336	1349	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03572	.	stop_codon	1342	1342	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03572	.	stem_loop	1316	1335	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03572	.	engineered_region	1309	1349	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03572	.	CDS	22	1212	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03572	.	CDS	22	1212	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03572	.	primer_binding_site	22	45	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03572	.	primer_binding_site	1193	1212	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03572	.	stop_codon	1210	1212	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03572	.	terminator	1221	1300	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03572	.	stem_loop	1232	1275	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03572	.	engineered_region	1221	1300	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03572	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03572	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03572	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03572	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_S03572
attaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtag
gcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatgg
cgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgc
cgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcc
tctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccga
tggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggg
ggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggct
gcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccg
gtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggca
gcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaa
tcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccgg
catggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattctt
ctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagc
ttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctc
ggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggt
gcatggagccgggccacctcgacctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgt
gaccgcattttg