LOCUS       BBa_S03581              2548 bp    DNA     linear       05-Dec-2025
DEFINITION  C0600:E0420
ACCESSION   BBa_S03581
VERSION     BBa_S03581.1  
FEATURES             Location/Qualifiers
     CDS             1..1662
                     /label=BBa_C0600
     misc_feature    1675..1678
                     /label=conserved
     RBS             1671..1682
                     /label=BBa_B0034
     CDS             1689..2411
                     /label=BBa_E0020
     stem_loop       2431..2474
                     /label=stem_loop
     misc_feature    2420..2499
                     /label=BBa_B0010
     terminator      2420..2499
                     /label=BBa_B0010
     stem_loop       2515..2534
                     /label=T7 TE
     misc_feature    2541..2541
                     /label=stop
     polyA_site      2535..2548
                     /label=polya
     misc_feature    2508..2548
                     /label=BBa_B0012
     terminator      2508..2548
                     /label=BBa_B0012
ORIGIN
        1 atgattaacc gtatccgcgt agtcacgctg ttggtaatgg tgctgggggt attcgcactg
       61 ttacagctta tttccggcag tctgtttttt tcttcccttc accatagcca gaagagcttt
      121 gtggtttcca atcaattacg ggaacagcag ggcgagctga cgtcaacctg ggatttaatg
      181 ctgcaaacgc gcattaacct gagtcgttca gcggtacgga tgatgatgga ttcctccaat
      241 caacaaagta acgccaaagt tgaattgctc gatagcgcca ggaaaacatt ggcgcaggca
      301 gcgacgcatt ataaaaaatt caaaagcatg gcaccgttac ctgaaatggt cgctaccagt
      361 cgtaatattg atgaaaaata taaaaactat tacacagcgt taactgaact gattgattac
      421 ctagattatg gcaatactgg agcttatttc gctcagccaa cccagggaat gcaaaatgca
      481 atgggcgaag cgtttgctca gtacgccctc agcagtgaaa aactgtatcg cgatatcgtc
      541 actgacaacg cagatgatta ccgatttgcc cagtggcaac tggcggttat cgcgctggtg
      601 gtggtattga ttctgctggt ggcgtggtac ggcattcgcc gtatgttgct tactccgctg
      661 gcaaaaatta ttgctcacat tcgcgaaatc gccggtggta acctggcgaa taccctgacc
      721 attgacgggc gcagtgaaat gggcgacctg gcgcagagcg tttcacatat gcaacgctct
      781 ttgactgaca ccgtcactca tgtccgcgaa ggttcagatg ccatctatgc cggtacccgt
      841 gaaattgcgg cgggcaacac cgatctttcc tcccgtactg aacagcaggc atccgcgctg
      901 gaagaaactg ccgccagcat ggagcagctc accgcgacag tgaagcaaaa cgccgataac
      961 gcccgccagg cctcgcaact ggcgcaaagt gcctccgaca ccgcccagca cggcggcaaa
     1021 gtggtggatg gcgtagtgaa aacgatgcat gagatcgccg atagttcgaa gaaaattgcc
     1081 gacattatca gcgttatcga cggtattgcc ttccagacta atatcctcgc gctgaatgcc
     1141 gcggttgaag ccgcgcgtgc gggtgaacag ggccgtggtt ttgccgtggt ggcgggtgaa
     1201 gtgcgtaatc ttgccagtcg cagcgcccag gcggcaaaag agatcaaagc cctcattgaa
     1261 gactccgtct cacgcgttga taccggttcg gtgctggtcg aaagcgccgg ggaaacaatg
     1321 aacaatatcg tcaatgctgt cactcgcgtg actgacatta tgggcgagat tgcatcggca
     1381 tcggatgaac agagccgtgg catcgatcaa gtcgcattgg cggtttcgga aatggatcgc
     1441 gtcacgcaac agaacgcatc gctggtgcag gaatcagctg ccgccgccgc tgcgctggaa
     1501 gaacaggcga gtcgtttaac gcaagcagtt tccgcgttcc gtctggcagc cagcccactc
     1561 accaataaac cgcaaacacc atcccgtcct gccagtgagc aaccaccggc tcagccacga
     1621 ctgcgaattg ctgaacaaga tccaaactgg gaaacatttt gatactagag aaagaggaga
     1681 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1741 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1801 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1861 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc
     1921 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1981 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2041 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2101 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc
     2161 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2221 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2281 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2341 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2401 acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2461 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2521 ccttcgggtg ggcctttctg cgtttata
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