LOCUS BBa_S03587 2477 bp DNA linear 25-Nov-2024 DEFINITION S0104:S03545 ACCESSION BBa_S03587 VERSION BBa_S03587.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..687 /label=cI 434 misc_feature 649..687 /label=LVA misc_feature 19..687 /label=BBa_C0052 misc_feature 688..712 /label=Help:Barcodes CDS 19..687 /label=BBa_C0052 misc_feature 725..728 /label=conserved RBS 721..732 /label=BBa_B0034 misc_feature 739..741 /label=start misc_feature 1522..1527 /label=2 misc_feature 1489..1521 /label=LVA CDS 739..1488 /label=aiiA misc_feature 739..1527 /label=BBa_C0060 misc_feature 1528..1552 /label=Help:Barcodes CDS 739..1527 /label=BBa_C0060 misc_feature 1565..1568 /label=conserved RBS 1561..1572 /label=BBa_B0034 CDS 1579..2340 /label=YFP (LVA) misc_feature 2335..2340 /label=2 misc_feature 2296..2334 /label=SsrA misc_feature 1820..1820 /label=A (Q->L) misc_feature 1579..2340 /label=BBa_E0032 misc_feature 1579..2340 /label=BBa_E0032 stem_loop 2360..2403 /label=stem_loop misc_feature 2349..2428 /label=BBa_B0010 terminator 2349..2428 /label=BBa_B0010 stem_loop 2444..2463 /label=T7 TE misc_feature 2470..2470 /label=stop polyA_site 2464..2477 /label=polya misc_feature 2437..2477 /label=BBa_B0012 terminator 2437..2477 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt 61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag 121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc 181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac 241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa 301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta 361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa 421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc 481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat 541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga 601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac 661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 721 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt 781 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg 841 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca 901 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta 961 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg 1021 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc 1081 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc 1141 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat 1201 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa 1261 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat 1321 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta 1381 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga 1441 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac 1501 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag 1561 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 1621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 1681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 1741 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 1801 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 1861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 1921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 2041 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 2101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 2161 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 2221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 2281 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 2341 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2401 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2461 gcctttctgc gtttata // LOCUS BBa_S03554 2477 bp DNA linear 25-Nov-2024 DEFINITION S0104:S03545 ACCESSION BBa_S03554 VERSION BBa_S03554.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..687 /label=cI 434 misc_feature 649..687 /label=LVA misc_feature 19..687 /label=BBa_C0052 misc_feature 688..712 /label=Help:Barcodes CDS 19..687 /label=BBa_C0052 misc_feature 1..712 /label=BBa_S0104 misc_feature 725..728 /label=conserved RBS 721..732 /label=BBa_B0034 misc_feature 739..741 /label=start misc_feature 1522..1527 /label=2 misc_feature 1489..1521 /label=LVA CDS 739..1488 /label=aiiA misc_feature 739..1527 /label=BBa_C0060 misc_feature 1528..1552 /label=Help:Barcodes CDS 739..1527 /label=BBa_C0060 misc_feature 721..1552 /label=BBa_C0260 misc_feature 1565..1568 /label=conserved RBS 1561..1572 /label=BBa_B0034 CDS 1579..2340 /label=YFP (LVA) misc_feature 2335..2340 /label=2 misc_feature 2296..2334 /label=SsrA misc_feature 1820..1820 /label=A (Q->L) misc_feature 1579..2340 /label=BBa_E0032 misc_feature 1579..2340 /label=BBa_E0032 stem_loop 2360..2403 /label=stem_loop misc_feature 2349..2428 /label=BBa_B0010 terminator 2349..2428 /label=BBa_B0010 stem_loop 2444..2463 /label=T7 TE misc_feature 2470..2470 /label=stop polyA_site 2464..2477 /label=polya misc_feature 2437..2477 /label=BBa_B0012 terminator 2437..2477 /label=BBa_B0012 misc_feature 1561..2477 /label=BBa_E0432 misc_feature 721..2477 /label=BBa_S03545 ORIGIN 1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt 61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag 121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc 181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac 241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa 301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta 361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa 421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc 481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat 541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga 601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac 661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 721 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt 781 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg 841 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca 901 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta 961 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg 1021 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc 1081 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc 1141 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat 1201 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa 1261 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat 1321 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta 1381 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga 1441 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac 1501 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag 1561 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 1621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 1681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 1741 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 1801 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 1861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 1921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 2041 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 2101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 2161 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 2221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 2281 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 2341 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2401 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2461 gcctttctgc gtttata //