LOCUS BBa_S03588 1922 bp DNA linear 22-Nov-2024 DEFINITION R0079:S03586 ACCESSION BBa_S03588 VERSION BBa_S03588.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 misc_feature 184..186 /label=start misc_feature 967..972 /label=2 misc_feature 934..966 /label=LVA CDS 184..933 /label=aiiA misc_feature 184..972 /label=BBa_C0060 misc_feature 973..997 /label=Help:Barcodes CDS 184..972 /label=BBa_C0060 misc_feature 1010..1013 /label=conserved RBS 1006..1017 /label=BBa_B0034 misc_feature 1780..1785 /label=stop misc_feature 1741..1779 /label=AAV misc_feature 1024..1785 /label=BBa_E0034 stem_loop 1805..1848 /label=stem_loop misc_feature 1794..1873 /label=BBa_B0010 terminator 1794..1873 /label=BBa_B0010 stem_loop 1889..1908 /label=T7 TE misc_feature 1915..1915 /label=stop polyA_site 1909..1922 /label=polya misc_feature 1882..1922 /label=BBa_B0012 terminator 1882..1922 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt ggatcattcg 241 tctgttaata gtacattaac accaggagaa ttattagact taccggtttg gtgttatctt 301 ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag tgcagttaat 361 aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa aatgactgaa 421 gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga ccttctttat 481 attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt tataaataca 541 ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga atatttgaaa 601 gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga agtcgtacca 661 ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct attaattgag 721 acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa agagaatttt 781 gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc aattaaacgt 841 ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga tatagagcag 901 gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa ctacgcttta 961 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac 1021 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 1081 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 1141 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 1201 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg 1261 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 1321 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 1381 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 1441 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 1501 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 1561 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 1621 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 1681 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 1741 aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta gagccaggca 1801 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1861 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1921 ta //