##gff-version 3
##sequence-region BBa_S03589 1 2477
BBa_S03589	.	engineered_region	721	2477	.	+	0	ID=BBa_S03586;Name=BBa_S03586
BBa_S03589	.	engineered_region	721	1552	.	+	0	ID=BBa_C0260;Name=BBa_C0260;Parent=BBa_S03586
BBa_S03589	.	CDS	739	1527	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_C0260
BBa_S03589	.	start_codon	739	741	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S03589	.	stop_codon	1522	1527	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S03589	.	tag	1489	1521	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S03589	.	CDS	739	1488	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S03589	.	engineered_tag	1528	1552	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S03589	.	engineered_region	739	1527	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S03589	.	ribosome_entry_site	721	732	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0260
BBa_S03589	.	conserved_region	725	728	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03589	.	engineered_region	1561	2477	.	+	0	ID=BBa_E0434;Name=BBa_E0434;Parent=BBa_S03586
BBa_S03589	.	ribosome_entry_site	1561	1572	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_S03589	.	conserved_region	1565	1568	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03589	.	terminator	2349	2428	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_S03589	.	stem_loop	2360	2403	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03589	.	engineered_region	2349	2428	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03589	.	engineered_region	1579	2340	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_S03589	.	stop_codon	2335	2340	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_S03589	.	tag	2296	2334	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_S03589	.	terminator	2437	2477	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_S03589	.	engineered_region	2437	2477	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03589	.	stop_codon	2470	2470	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03589	.	polyA_site	2464	2477	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03589	.	stem_loop	2444	2463	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03589	.	engineered_region	1	712	.	+	0	ID=BBa_S0104;Name=BBa_S0104
BBa_S03589	.	CDS	19	687	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_S0104
BBa_S03589	.	engineered_tag	688	712	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_S03589	.	engineered_region	19	687	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_S03589	.	tag	649	687	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_S03589	.	CDS	19	687	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_S03589	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0104
BBa_S03589	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03589
aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaac
ttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttacc
agaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttggg
cacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacg
atatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtga
ttccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaat
ggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagt
acctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagc
gagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatc
attcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaaga
aggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaa
gggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagacc
ttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgt
acagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaa
atcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgc
tattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatga
agtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaag
ccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaa
gctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaa
gaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata