LOCUS       BBa_S03595              1349 bp    DNA     linear       16-Feb-2025
DEFINITION  TT : RBS-TetF
ACCESSION   BBa_S03595
VERSION     BBa_S03595.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     RBS             138..152
                     /label=RBS-1\Strong
     RBS             145..149
                     /label=RBS
     misc_feature    138..152
                     /label=BBa_B0030
     RBS             138..152
                     /label=BBa_B0030
     CDS             159..1349
                     /label=tetA(C) forward
     primer_bind     159..182
                     /label=Fwd primer J31007
     primer_bind     1330..1349
                     /label=Rev primer J31007
     misc_feature    1347..1349
                     /label=stop
     CDS             159..1349
                     /label=BBa_J31007
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagatt aaagaggaga aatactagat gaaatctaac aatgcgctca
      181 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
      241 tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc
      301 tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg
      361 accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg
      421 cgatcatggc gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca
      481 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      541 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      601 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      661 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      721 agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
      781 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
      841 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
      901 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
      961 gccccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     1021 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     1081 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     1141 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     1201 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     1261 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     1321 gtgcatggag ccgggccacc tcgacctaa
//