LOCUS BBa_S03596 2899 bp DNA linear 22-Nov-2024 DEFINITION AraCPCrev-TT : pBad-HixC-RBS-TetF-HixC-TT-RE ACCESSION BBa_S03596 VERSION BBa_S03596.1 FEATURES Location/Qualifiers misc_feature 1..1114 /label=BBa_I13458 stem_loop 1134..1177 /label=stem_loop misc_feature 1123..1202 /label=BBa_B0010 terminator 1123..1202 /label=BBa_B0010 stem_loop 1218..1237 /label=T7 TE misc_feature 1244..1244 /label=stop polyA_site 1238..1251 /label=polya misc_feature 1211..1251 /label=BBa_B0012 terminator 1211..1251 /label=BBa_B0012 promoter 1260..1389 /label=BBa_I13453 misc_feature 1398..1423 /label=BBa_J44000 RBS 1432..1446 /label=RBS-1\Strong RBS 1439..1443 /label=RBS misc_feature 1432..1446 /label=BBa_B0030 RBS 1432..1446 /label=BBa_B0030 CDS 1453..2643 /label=tetA(C) forward primer_bind 1453..1476 /label=Fwd primer J31007 primer_bind 2624..2643 /label=Rev primer J31007 misc_feature 2641..2643 /label=stop CDS 1453..2643 /label=BBa_J31007 misc_feature 2652..2677 /label=BBa_J44000 stem_loop 2697..2740 /label=stem_loop misc_feature 2686..2765 /label=BBa_B0010 terminator 2686..2765 /label=BBa_B0010 stem_loop 2781..2800 /label=T7 TE misc_feature 2807..2807 /label=stop polyA_site 2801..2814 /label=polya misc_feature 2774..2814 /label=BBa_B0012 terminator 2774..2814 /label=BBa_B0012 misc_feature 2873..2878 /label=Former SpeI site misc_feature 2873..2873 /label=Mutation of SpeI site misc_feature 2823..2899 /label=RE Sequence misc_feature 2851..2851 /label=Originally a C misc_feature 2899..2899 /label=Insertion right before biobrick ends misc_feature 2828..2843 /label=Proximal Fis Binding Site misc_feature 2877..2891 /label=Distal Fis Binding Site misc_feature 2823..2899 /label=BBa_J3101 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctattactag agccaggcat caaataaaac 1141 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1201 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 1261 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1321 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 1381 tgggctagct actagagtta tcaaaaacca tggtttttga taatactaga gattaaagag 1441 gagaaatact agatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg 1501 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc 1561 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa 1621 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg 1681 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg 1741 tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct 1801 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg 1861 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc 1921 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg 1981 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc 2041 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 2101 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc 2161 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga 2221 atcttgcacg ccctcgctca agccttcgtc actggccccg ccaccaaacg tttcggcgag 2281 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc 2341 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg 2401 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt 2461 caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg 2521 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc 2581 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc 2641 taatactaga gttatcaaaa accatggttt ttgataatac tagagccagg catcaaataa 2701 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2761 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2821 agttcgggtg tcaacaattg accaaaatat tgatttacag cgtaatgcgc tttctagtgc 2881 aaattgtgac cgcattttg //