LOCUS       BBa_S03603              1814 bp    DNA     linear       15-Jun-2024
DEFINITION  S03600:S03574
ACCESSION   BBa_S03603
VERSION     BBa_S03603.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     misc_feature    874..877
                     /label=conserved
     RBS             870..881
                     /label=BBa_B0034
     CDS             888..1550
                     /label=CinI
     misc_feature    1551..1583
                     /label=LVA
     misc_feature    1590..1614
                     /label=Help:Barcodes
     CDS             888..1589
                     /label=BBa_C0076
     stem_loop       1634..1677
                     /label=stem_loop
     misc_feature    1623..1702
                     /label=BBa_B0010
     terminator      1623..1702
                     /label=BBa_B0010
     stem_loop       1718..1737
                     /label=T7 TE
     misc_feature    1744..1744
                     /label=stop
     polyA_site      1738..1751
                     /label=polya
     misc_feature    1711..1751
                     /label=BBa_B0012
     terminator      1711..1751
                     /label=BBa_B0012
     misc_feature    1760..1779
                     /label=LuxR/HSL
     promoter        1779..1784
                     /label=-35
     promoter        1801..1806
                     /label=-10
     misc_feature    1812..1812
                     /label=start
     misc_feature    1760..1814
                     /label=BBa_R0062
     promoter        1760..1814
                     /label=BBa_R0062
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg ttcgttatca
      901 tccaggctca cgaataccag aaatacgctg ctgttctgga ccagatgttc cgtctgcgta
      961 aaaaagtttt cgctgacacc ctgtgctggg acgttccggt tatcggtccg tacgaacgtg
     1021 actcctacga ctccctggct ccggcttacc tggtttggtg caacgactcc cgtacccgtc
     1081 tgtacggtgg tatgcgtctg atgccgacca ccggtccgac cctgctgtac gacgttttcc
     1141 gtgaaacctt cccggacgct gctgacctga tcgctccggg tatctgggaa ggtacccgta
     1201 tgtgcatcga cgaagaagct atcgctaaag acttcccgga aatcgacgct ggtcgtgctt
     1261 tctccatgat gctgctggct ctgtgcgaat gcgctctgga ccacggtatc cacaccatga
     1321 tctccaacta cgaaccgtac ctgaaacgtg tttacaaacg tgctggtgct gaagttgaag
     1381 aactgggtcg tgctgacggt tacggtaaat acccggtttg ctgcggtgct ttcgaagttt
     1441 ccgaccgtgt tctgcgtaaa atgcgtgctg ctctgggtct gaccctgccg ctgtacgttc
     1501 gtcacgttcc ggctcgttcc gttgttaccc agttcctgga aatggctgct gctgctaacg
     1561 acgaaaacta cgctctggtt gcttaataac cctgatagtg ctagtgtaga tccctactag
     1621 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1681 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1741 ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt
     1801 tatagtcgaa taaa
//