LOCUS       BBa_S03609              2198 bp    DNA     linear       25-Nov-2024
DEFINITION  AraCPCrev-TT : pLac-RBS-HinLVA-TT
ACCESSION   BBa_S03609
VERSION     BBa_S03609.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     stem_loop       1134..1177
                     /label=stem_loop
     misc_feature    1123..1202
                     /label=BBa_B0010
     terminator      1123..1202
                     /label=BBa_B0010
     stem_loop       1218..1237
                     /label=T7 TE
     misc_feature    1244..1244
                     /label=stop
     polyA_site      1238..1251
                     /label=polya
     misc_feature    1211..1251
                     /label=BBa_B0012
     terminator      1211..1251
                     /label=BBa_B0012
     CDS             1260..1347
                     /label=end of LacI coding region (inactive)
     misc_feature    1260..1459
                     /label=BBa_R0010
     misc_feature    1348..1385
                     /label=CAP binding site
     promoter        1396..1401
                     /label=-35
     promoter        1420..1425
                     /label=-10
     misc_feature    1425..1459
                     /label=LacI binding site
     misc_feature    1432..1432
                     /label=start
     promoter        1260..1459
                     /label=BBa_R0010
     RBS             1468..1482
                     /label=RBS-1\Strong
     RBS             1475..1479
                     /label=RBS
     misc_feature    1468..1482
                     /label=BBa_B0030
     RBS             1468..1482
                     /label=BBa_B0030
     misc_feature    1558..1563
                     /label=Former SpeI site
     misc_feature    1560..1560
                     /label=Mutation to delete the SpeI site
     CDS             1489..2061
                     /label=Hin sequence
     primer_bind     1489..1491
                     /label=Fwd primer J31001
     primer_bind     2039..2058
                     /label=Rev primer J31001
     CDS             1489..2061
                     /label=BBa_J31000
     stem_loop       2081..2124
                     /label=stem_loop
     misc_feature    2070..2149
                     /label=BBa_B0010
     terminator      2070..2149
                     /label=BBa_B0010
     stem_loop       2165..2184
                     /label=T7 TE
     misc_feature    2191..2191
                     /label=stop
     polyA_site      2185..2198
                     /label=polya
     misc_feature    2158..2198
                     /label=BBa_B0012
     terminator      2158..2198
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agccaggcat caaataaaac
     1141 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1201 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc
     1261 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1321 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1381 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1441 cggataacaa tttcacacat actagagatt aaagaggaga aatactagat ggctactatt
     1501 gggtatattc gggtgtcaac aattgaccaa aatatcgatt tacagcgtaa tgcgcttacc
     1561 agtgcaaatt gtgaccgcat ttttgaagac cgtatcagtg gcaagattgc aaaccgcccc
     1621 ggcctgaaac gggcgttaaa gtatgtaaat aaaggcgata ctcttgtcgt ctggaaatta
     1681 gacagactgg gccgtagcgt gaaaaatctg gtggcgttaa tatcagaatt acatgaacgt
     1741 ggagctcact tccattcttt aaccgatagt attgatacca gtagcgcgat ggggcgattc
     1801 ttttttcatg taatgtcagc actggccgag atggagcgag aattaatcgt cgagcgaacc
     1861 cttgccggac tggctgccgc cagagcgcaa ggacgactgg gagggcgccc tcgggcgatc
     1921 aacaaacatg aacaggaaca gattagtcgg ctattagaga aaggccatcc tcggcagcaa
     1981 ttagctatta tttttggtat tggcgtatcc accttataca gatactttcc ggcaagcagt
     2041 ataaaaaaac gaatgaatta atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2101 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2161 cactggctca ccttcgggtg ggcctttctg cgtttata
//