LOCUS BBa_S03614 5878 bp DNA linear 22-Nov-2024 DEFINITION J13002:J32007 ACCESSION BBa_S03614 VERSION BBa_S03614.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..5878 /label=J32007 CDS 81..5878 /label= Photorhabdus luminescens LuxCDABE CDS 81..1524 /label=LuxC CDS 1535..2458 /label=LuxD CDS 2507..3589 /label=LuxA CDS 3604..4587 /label=LuxB CDS 4766..5878 /label=LuxE CDS 81..5878 /label=BBa_J32007 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgactaaaa aaatttcatt cattattaac ggccaggttg 121 aaatctttcc cgaaagtgat gatttagtgc aatccattaa ttttggtgat aatagtgttt 181 acctgccaat attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg 241 aattacggtt gcataacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg 301 aagaatactc aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag 361 aagaaatggc taagctagag gccaattgga tatctatgat tttatgttct aaaggcggcc 421 tttatgatgt tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc 481 aggatgaaag ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta 541 atgttccatt atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta 601 ttataaaaac atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg 661 atgtagaccc taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag 721 gtgatacatc actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag 781 ggccagatgc gattaattgg gcggtagagc atgcgccatc ttatgctgat gtgattaaat 841 ttggttctaa aaagagtctt tgcattatcg ataatcctgt tgatttgacg tccgcagcga 901 caggtgcggc tcatgatgtt tgtttttacg atcagcgagc ttgtttttct gcccaaaaca 961 tatattacat gggaaatcat tatgaggaat ttaagttagc gttgatagaa aaacttaatc 1021 tatatgcgca tatattaccg aatgccaaaa aagattttga tgaaaaggcg gcctattctt 1081 tagttcaaaa agaaagcttg tttgctggat taaaagtaga ggtggatatt catcaacgtt 1141 ggatgattat tgagtcaaat gcaggtgtgg aatttaatca accacttggc agatgtgtgt 1201 accttcatca cgtcgataat attgagcaaa tattgcctta tgttcaaaaa aataagacgc 1261 aaaccatatc tatttttcct tgggagtcat catttaaata tcgagatgcg ttagcattaa 1321 aaggtgcgga aaggattgta gaagcaggaa tgaataacat atttcgagtt ggtggatctc 1381 atgacggaat gagaccgttg caacgattag tgacatatat ttctcatgaa aggccatcta 1441 actatacggc taaggatgtt gcggttgaaa tagaacagac tcgattcctg gaagaagata 1501 agttccttgt atttgtccca taataggtaa aagtatggaa aatgaatcaa aatataaaac 1561 catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc 1621 agaagaaaac agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag 1681 gatggatcat tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg 1741 ctatgattcg cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc 1801 tataggaaag cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa 1861 cttcggtatg ttggcttcaa gcttatctgc gcggatagct tatgcaagcc tatctgaaat 1921 caatgcttcg tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag 1981 agctttaggg tttgattatc tcagtctacc cattaatgaa ttgccggata acctagattt 2041 tgaaggccat aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga 2101 agatttagct tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac 2161 tgcaaataac gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg 2221 tagtaatcga tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt 2281 agtggtcctg cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga 2341 tcatctggat attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac 2401 agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa 2461 tctattgaga tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa 2521 ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt 2581 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca 2641 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc 2701 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg 2761 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat 2821 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg 2881 cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga 2941 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag 3001 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca 3061 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact 3121 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg 3181 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa 3241 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga 3301 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca 3361 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca 3421 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg 3481 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc 3541 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa 3601 gaaatgaaat ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa 3661 agtatagttc gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt 3721 ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct 3781 ggttttctgc tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca 3841 actcatcatc ctgtcgccat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg 3901 agatttattt tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc 3961 ccggttgaat atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta 4021 acaacaggct attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat 4081 ccccatgctt atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt 4141 gttgagtggg cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat 4201 gttagatatg aatatgctga aagatataaa gccgttgcgg ataaatatga cgttgaccta 4261 tcagagatag accatcagtt aatgatatta gttaactata acgaagatag taataaagct 4321 aaacaagaga cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat 4381 ttcgaaaata aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt 4441 ataactgcgg ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt 4501 gaaccaatga atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt 4561 aagaagtacc acatggaata tacctaatag atttcgagtt gcagcgaggc ggcaagtgaa 4621 cgaatcccca ggagcataga taactatgtg actggggtga gtgaaagcag ccaacaaagc 4681 agcagcttga aagatgaagg gtataaaaga gtatgacagc agtgctgcca tactttctaa 4741 tattatcttg aggagtaaaa caggtatgac ttcatatgtt gataaacaag aaattacagc 4801 aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt cttacgacga 4861 gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt ataaacattg 4921 tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta cggaaattga 4981 tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa cttctcagga 5041 aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa gtcaggtggc 5101 gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca tgaaatatgt 5161 tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata gatttaatgc 5221 tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc ctacgacatt 5281 taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg aacgaataaa 5341 aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt tactctgcca 5401 ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata tcataaccgg 5461 aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca atcatctttt 5521 atttgatact ttcaatctca gtgatattag tcagatccga gatatattta atcaagttga 5581 actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc cgtgggtata 5641 tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc cggggttgat 5701 gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg atgatgtcgg 5761 gataattagc agagaatatg gtaagtatcc cggcgtgctc gttgaaattt tacgtcgcgt 5821 caatacgagg acgcagaaag ggtgtgcttt aagcttaacc gaagcgtttg atagttga //