LOCUS BBa_S03617 1091 bp DNA linear 23-Dec-2024 DEFINITION S03597:J32015 ACCESSION BBa_S03617 VERSION BBa_S03617.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 stem_loop 820..863 /label=stem_loop misc_feature 809..888 /label=BBa_B0010 terminator 809..888 /label=BBa_B0010 stem_loop 904..923 /label=T7 TE misc_feature 930..930 /label=stop polyA_site 924..937 /label=polya misc_feature 897..937 /label=BBa_B0012 terminator 897..937 /label=BBa_B0012 misc_feature 946..964 /label=TetR 1 misc_feature 946..999 /label=BBa_R0040 promoter 965..970 /label=-35 misc_feature 971..989 /label=TetR 2 promoter 988..993 /label=-10 promoter 946..999 /label=BBa_R0040 misc_feature 1012..1015 /label=conserved RBS 1008..1019 /label=BBa_B0034 misc_feature 1026..1091 /label=PelB misc_feature 1026..1091 /label=BBa_J32015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 901 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata 961 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat 1021 actagatgaa atacctgctg ccgaccgctg ctgctggtct gctgctcctc gctgcccagc 1081 cggcgatggc c // LOCUS BBa_S03602 1091 bp DNA linear 23-Dec-2024 DEFINITION S03597:J32015 ACCESSION BBa_S03602 VERSION BBa_S03602.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 stem_loop 820..863 /label=stem_loop misc_feature 809..888 /label=BBa_B0010 terminator 809..888 /label=BBa_B0010 stem_loop 904..923 /label=T7 TE misc_feature 930..930 /label=stop polyA_site 924..937 /label=polya misc_feature 897..937 /label=BBa_B0012 terminator 897..937 /label=BBa_B0012 misc_feature 946..964 /label=TetR 1 misc_feature 946..999 /label=BBa_R0040 promoter 965..970 /label=-35 misc_feature 971..989 /label=TetR 2 promoter 988..993 /label=-10 promoter 946..999 /label=BBa_R0040 misc_feature 1012..1015 /label=conserved RBS 1008..1019 /label=BBa_B0034 misc_feature 1026..1091 /label=PelB misc_feature 1026..1091 /label=BBa_J32015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 901 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata 961 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat 1021 actagatgaa atacctgctg ccgaccgctg ctgctggtct gctgctcctc gctgcccagc 1081 cggcgatggc c //