BBa_S03617
type: ComponentDefinition
persistent identity: BBa S03617
display id: BBa_S03617
version: 1
was generated by: iGEM to SBOL conversion
description: S03597:J32015
created: 2006-10-18T11:00:00Z
modified: 2015-05-08T01:14:25Z
owned by: james
owned by: myers
discontinued: true
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
is replaced by: BBa_S03602
creator: Austen Heinz
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_J32015
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J32015
sequence: https://synbiohub.org/public/igem/BBa_S03617_sequence/1

BBa_B0034
type: Component
persistent identity: component1903626
display id: component1903626
version: 1
top level: BBa_S03617
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1903621
display id: component1903621
version: 1
top level: BBa_S03617
definition: p(tetR)
access: public

BBa_J32015
type: Component
persistent identity: component1903628
display id: component1903628
version: 1
top level: BBa_S03617
definition: PelB
access: public

BBa_R0040
type: Component
persistent identity: component1903607
display id: component1903607
version: 1
top level: BBa_S03617
definition: p(tetR)
access: public

BBa_E0040
type: Component
persistent identity: component1903613
display id: component1903613
version: 1
top level: BBa_S03617
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1903612
display id: component1903612
version: 1
top level: BBa_S03617
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1903616
display id: component1903616
version: 1
top level: BBa_S03617
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1903614
display id: component1903614
version: 1
top level: BBa_S03617
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1903607
display id: annotation1903607
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903607/range1903607/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03617/annotation1903607/range1903607/1
type: Range
persistent identity: range1903607
display id: range1903607
version: 1
top level: BBa_S03617
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1903612
display id: annotation1903612
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903612/range1903612/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03617/annotation1903612/range1903612/1
type: Range
persistent identity: range1903612
display id: range1903612
version: 1
top level: BBa_S03617
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1903613
display id: annotation1903613
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903613/range1903613/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03617/annotation1903613/range1903613/1
type: Range
persistent identity: range1903613
display id: range1903613
version: 1
top level: BBa_S03617
direction: direction/reverse
start: 81
end: 800
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1903614
display id: annotation1903614
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903614/range1903614/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03617/annotation1903614/range1903614/1
type: Range
persistent identity: range1903614
display id: range1903614
version: 1
top level: BBa_S03617
direction: direction/forward
start: 809
end: 888
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1903616
display id: annotation1903616
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903616/range1903616/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03617/annotation1903616/range1903616/1
type: Range
persistent identity: range1903616
display id: range1903616
version: 1
top level: BBa_S03617
direction: direction/forward
start: 897
end: 937
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1903621
display id: annotation1903621
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903621/range1903621/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03617/annotation1903621/range1903621/1
type: Range
persistent identity: range1903621
display id: range1903621
version: 1
top level: BBa_S03617
direction: direction/forward
start: 946
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1903626
display id: annotation1903626
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903626/range1903626/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03617/annotation1903626/range1903626/1
type: Range
persistent identity: range1903626
display id: range1903626
version: 1
top level: BBa_S03617
direction: direction/reverse
start: 1008
end: 1019
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation1903628
display id: annotation1903628
version: 1
top level: BBa_S03617
location: https://synbiohub.org/public/igem/BBa_S03617/annotation1903628/range1903628/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_S03617/annotation1903628/range1903628/1
type: Range
persistent identity: range1903628
display id: range1903628
version: 1
top level: BBa_S03617
direction: direction/reverse
start: 1026
end: 1091
orientation: inline

BBa_S03602
type: ComponentDefinition
persistent identity: BBa S03602
display id: BBa_S03602
version: 1
was generated by: iGEM to SBOL conversion
description: S03597:J32015
created: 2006-09-27T11:00:00Z
modified: 2015-05-08T01:14:25Z
owned by: james
owned by: myers
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Austen Heinz
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_J32015
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J32015
sequence: https://synbiohub.org/public/igem/BBa_S03602_sequence/1

BBa_E0040
type: Component
persistent identity: component1902427
display id: component1902427
version: 1
top level: BBa_S03602
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1902426
display id: component1902426
version: 1
top level: BBa_S03602
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1902430
display id: component1902430
version: 1
top level: BBa_S03602
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1902428
display id: component1902428
version: 1
top level: BBa_S03602
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1902440
display id: component1902440
version: 1
top level: BBa_S03602
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1902435
display id: component1902435
version: 1
top level: BBa_S03602
definition: p(tetR)
access: public

BBa_J32015
type: Component
persistent identity: component1902442
display id: component1902442
version: 1
top level: BBa_S03602
definition: PelB
access: public

BBa_R0040
type: Component
persistent identity: component1902421
display id: component1902421
version: 1
top level: BBa_S03602
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1902421
display id: annotation1902421
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902421/range1902421/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03602/annotation1902421/range1902421/1
type: Range
persistent identity: range1902421
display id: range1902421
version: 1
top level: BBa_S03602
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1902426
display id: annotation1902426
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902426/range1902426/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03602/annotation1902426/range1902426/1
type: Range
persistent identity: range1902426
display id: range1902426
version: 1
top level: BBa_S03602
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1902427
display id: annotation1902427
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902427/range1902427/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03602/annotation1902427/range1902427/1
type: Range
persistent identity: range1902427
display id: range1902427
version: 1
top level: BBa_S03602
direction: direction/reverse
start: 81
end: 800
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1902428
display id: annotation1902428
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902428/range1902428/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03602/annotation1902428/range1902428/1
type: Range
persistent identity: range1902428
display id: range1902428
version: 1
top level: BBa_S03602
direction: direction/forward
start: 809
end: 888
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1902430
display id: annotation1902430
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902430/range1902430/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03602/annotation1902430/range1902430/1
type: Range
persistent identity: range1902430
display id: range1902430
version: 1
top level: BBa_S03602
direction: direction/forward
start: 897
end: 937
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1902435
display id: annotation1902435
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902435/range1902435/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03602/annotation1902435/range1902435/1
type: Range
persistent identity: range1902435
display id: range1902435
version: 1
top level: BBa_S03602
direction: direction/forward
start: 946
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1902440
display id: annotation1902440
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902440/range1902440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03602/annotation1902440/range1902440/1
type: Range
persistent identity: range1902440
display id: range1902440
version: 1
top level: BBa_S03602
direction: direction/reverse
start: 1008
end: 1019
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation1902442
display id: annotation1902442
version: 1
top level: BBa_S03602
location: https://synbiohub.org/public/igem/BBa_S03602/annotation1902442/range1902442/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_S03602/annotation1902442/range1902442/1
type: Range
persistent identity: range1902442
display id: range1902442
version: 1
top level: BBa_S03602
direction: direction/reverse
start: 1026
end: 1091
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: iGEM to SBOL conversion
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: In stock
status: status/Available
bookmark: true
mutable notes: .
star: true
creator: Austen Heinz
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
top level: PelB
direction: direction/forward
start: 1
end: 66
orientation: inline

https://synbiohub.org/public/igem/BBa_S03617_sequence/1
type: Sequence
persistent identity: BBa S03617 sequence
display id: BBa_S03617_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03602_sequence/1
type: Sequence
persistent identity: BBa S03602 sequence
display id: BBa_S03602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32015_sequence/1
type: Sequence
persistent identity: BBa J32015 sequence
display id: BBa_J32015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z