LOCUS       BBa_S03621              1069 bp    DNA     linear       22-Dec-2024
DEFINITION  B0015:S03607
ACCESSION   BBa_S03621
VERSION     BBa_S03621.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    140..156
                     /label=lac O1
     promoter        157..162
                     /label=-35
     misc_feature    163..179
                     /label=lac O1
     promoter        180..185
                     /label=-10
     misc_feature    138..191
                     /label=BBa_R0011
     promoter        138..191
                     /label=BBa_R0011
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             219..929
                     /label=mCherry
     misc_feature    570..570
                     /label=C->T (removing PstI site)
     misc_feature    219..932
                     /label=BBa_J06504
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag
      181 atactgagca catactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      241 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      301 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      361 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      421 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      481 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      541 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      601 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      661 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      721 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      781 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      841 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      901 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//