##gff-version 3
##sequence-region BBa_S03621 1 1069
BBa_S03621	.	terminator	1029	1069	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03621	.	stop_codon	1062	1062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03621	.	polyA_site	1056	1069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03621	.	stem_loop	1036	1055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03621	.	engineered_region	1029	1069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03621	.	terminator	941	1020	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03621	.	engineered_region	941	1020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03621	.	stem_loop	952	995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03621	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03621	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03621	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03621	.	promoter	138	191	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S03621	.	promoter	180	185	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S03621	.	engineered_region	138	191	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S03621	.	operator	140	156	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S03621	.	promoter	157	162	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S03621	.	operator	163	179	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S03621	.	engineered_region	219	932	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_S03621	.	sequence_alteration	570	570	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_S03621	.	CDS	219	929	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_S03621	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03621	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03621	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03621	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03621	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03621	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03621	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03621
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaatt
gacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgagg
aggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcga
gatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctg
cccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccg
actacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgt
gacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggc
cccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcg
agatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcc
cgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaa
cagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata