LOCUS BBa_J58107 1711 bp DNA linear 22-Dec-2024 DEFINITION Trg-EnvZ regulated by LacI without terminator ACCESSION BBa_J58107 VERSION BBa_J58107.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1711 /label=BBa_J58104 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atacaactcc 241 ctcacagcga ttaggttttt tgcatcacat caggttggtt ccgttatttg cctgcattct 301 aggcggtatc ttagttctat tcgcattaag ttcagccctg gctggctatt tcctctggca 361 ggccgatcgc gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa 421 cagttcagat tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagtcg 481 tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca 541 gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc 601 cctcgacact gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt 661 gaaatatgcc aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg 721 accgctggat aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag 781 agccaaccaa ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat 841 tggcgcgttt gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat 901 cgtcattcgt ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct 961 gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca 1021 gcatatggcg gctggtgtta agcaactggc ggatgaccgc acgctgctga tggcgggggt 1081 aagtcacgac ttgcgcacgc cgctgacgcg tattcgcctg gcgactgaga tgatgagcga 1141 gcaggatggc tatctggcag aatcgatcaa taaagatatc gaagagtgca acgccatcat 1201 tgagcagttt atcgactacc tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct 1261 taatgcagta ctcggtgagg tgattgctgc cgaaagtggc tatgagcggg aaattgaaac 1321 cgcgctttac cccggcagca ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt 1381 ggcgaatatg gtggtcaacg ccgcccgtta tggcaatggc tggatcaaag tcagcagcgg 1441 aacggagccg aatcgcgcct ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga 1501 acaacgtaag cacctgttcc agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg 1561 cacgggatta gggctggcaa ttgtgcagcg tatcgtggat aaccataacg ggatgctgga 1621 gcttggcacc agcgagcggg gcgggctttc cattcgcgcc tggctgccag tgccggtaac 1681 gcgggcgcag ggcacgacaa aagaagggta a // LOCUS BBa_S03627 1711 bp DNA linear 22-Dec-2024 DEFINITION J04500:J58104 ACCESSION BBa_S03627 VERSION BBa_S03627.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1711 /label=BBa_J58104 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atacaactcc 241 ctcacagcga ttaggttttt tgcatcacat caggttggtt ccgttatttg cctgcattct 301 aggcggtatc ttagttctat tcgcattaag ttcagccctg gctggctatt tcctctggca 361 ggccgatcgc gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa 421 cagttcagat tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagtcg 481 tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca 541 gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc 601 cctcgacact gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt 661 gaaatatgcc aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg 721 accgctggat aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag 781 agccaaccaa ctggcggaac tggcccatca gcgcacccgc ctgggtggga tgttcatgat 841 tggcgcgttt gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat 901 cgtcattcgt ccactgcaac atgccgcaca acggattgaa aaaatcgcca gtggcgatct 961 gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca 1021 gcatatggcg gctggtgtta agcaactggc ggatgaccgc acgctgctga tggcgggggt 1081 aagtcacgac ttgcgcacgc cgctgacgcg tattcgcctg gcgactgaga tgatgagcga 1141 gcaggatggc tatctggcag aatcgatcaa taaagatatc gaagagtgca acgccatcat 1201 tgagcagttt atcgactacc tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct 1261 taatgcagta ctcggtgagg tgattgctgc cgaaagtggc tatgagcggg aaattgaaac 1321 cgcgctttac cccggcagca ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt 1381 ggcgaatatg gtggtcaacg ccgcccgtta tggcaatggc tggatcaaag tcagcagcgg 1441 aacggagccg aatcgcgcct ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga 1501 acaacgtaag cacctgttcc agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg 1561 cacgggatta gggctggcaa ttgtgcagcg tatcgtggat aaccataacg ggatgctgga 1621 gcttggcacc agcgagcggg gcgggctttc cattcgcgcc tggctgccag tgccggtaac 1681 gcgggcgcag ggcacgacaa aagaagggta a //