##gff-version 3
##sequence-region BBa_S03635 1 1674
BBa_S03635	.	promoter	35	164	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_S03635	.	sequence_feature	1598	1674	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_S03635	.	sequence_feature	1674	1674	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_S03635	.	sequence_feature	1598	1674	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_S03635	.	sequence_feature	1648	1653	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_S03635	.	sequence_feature	1648	1648	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_S03635	.	non_covalent_binding_site	1652	1666	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_S03635	.	sequence_feature	1626	1626	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_S03635	.	non_covalent_binding_site	1603	1618	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_S03635	.	sequence_feature	173	198	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03635	.	sequence_feature	1427	1452	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03635	.	CDS	228	1418	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03635	.	CDS	228	1418	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03635	.	primer_binding_site	1399	1418	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03635	.	primer_binding_site	228	251	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03635	.	stop_codon	1416	1418	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03635	.	sequence_feature	1	26	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03635	.	terminator	1461	1540	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03635	.	stem_loop	1472	1515	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03635	.	engineered_region	1461	1540	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03635	.	terminator	1549	1589	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03635	.	stem_loop	1556	1575	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03635	.	stop_codon	1582	1582	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03635	.	engineered_region	1549	1589	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03635	.	polyA_site	1576	1589	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03635	.	ribosome_entry_site	207	221	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03635	.	ribosome_entry_site	214	218	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03635	.	engineered_region	207	221	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03635	.	ribosome_entry_site	207	221	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_S03635
ttatcaaaaaccatggtttttgataatactagagacattgattatttgcacggcgtcacactttgctatgccatagcat
ttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgg
gctagctactagagttatcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatcta
acaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccggg
cctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaa
tttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggag
ccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcac
cggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctc
atgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcac
cattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggaga
gcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca
cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgct
ttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcac
tggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttg
ctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgt
tgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcct
aacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggatt
gtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatact
agagttatcaaaaaccatggtttttgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaat
tgtgaccgcattttg