BBa_J44000
1
hixC
hixC binding site for Salmonella typhimurium Hin recombinase
2006-06-05T11:00:00Z
2015-08-31T04:08:48Z
Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase
[http://www.genetics.org/cgi/content/abstract/149/4/1649]
A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
false
true
_71_
0
606
61
In stock
true
Standard BioBrick prefix and suffix were added to the 26 bp sequence.
true
Missouri Western and Davidson Groups, Todd Eckdahl
BBa_S03636
1
BBa_S03636
TetB-RBS<sub>rev</sub> : HixC
2006-10-27T11:00:00Z
2015-05-08T01:14:26Z
false
true
_61_
0
1144
61
It's complicated
false
false
Karmella Haynes
component2219352
1
BBa_S03532
component2219353
1
BBa_J44000
annotation2219353
1
BBa_J44000
range2219353
1
1223
1248
annotation2219352
1
BBa_S03532
range2219352
1
1
1214
BBa_J44001
1
BBa_J44001
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
2006-08-01T11:00:00Z
2015-08-31T04:08:48Z
Cloned from synthetic oligos
Released HQ 2013
Reverse version of RBS BBa_B0030
false
false
_61_71_
0
606
61
In stock
true
Repeats in oligos caused unusual products during cloning
true
Todd Eckdahl
annotation1893199
1
Reverse RBS
range1893199
1
1
15
BBa_S03532
1
BBa_S03532
TetB-RBS<sub>rev</sub>
2006-08-01T11:00:00Z
2015-05-08T01:14:24Z
false
false
_61_
0
919
61
It's complicated
false
false
Davidson and Missouri Western
component1932652
1
BBa_J44001
component1932650
1
BBa_J31006
annotation1932650
1
BBa_J31006
range1932650
1
1
1191
annotation1932652
1
BBa_J44001
range1932652
1
1200
1214
BBa_J31006
1
tetA(C)b
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
2006-07-11T11:00:00Z
2015-08-31T04:08:45Z
PsB1AT3
This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
false
false
_61_
0
919
61
It's complicated
true
It was cloned into PsB1A2 plasmids.
true
Sabriya Rosemond, Erin Zwack
annotation1910575
1
Rev primer J31006
range1910575
1
1
20
annotation1910574
1
stop
range1910574
1
1
3
annotation1884998
1
tetA(C) backwards
range1884998
1
1
1191
annotation1910576
1
Fwd primer J31006
range1910576
1
1168
1191
BBa_J31006_sequence
1
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
BBa_S03532_sequence
1
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaat
BBa_J44001_sequence
1
tttctcctctttaat
BBa_S03636_sequence
1
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataa
BBa_J44000_sequence
1
ttatcaaaaaccatggtttttgataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z