LOCUS       BBa_S03646              2894 bp    DNA     linear       23-Dec-2024
DEFINITION  S03635:S03562
ACCESSION   BBa_S03646
VERSION     BBa_S03646.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     promoter        35..164
                     /label=BBa_I13453
     misc_feature    173..198
                     /label=BBa_J44000
     RBS             207..221
                     /label=RBS-1\Strong
     RBS             214..218
                     /label=RBS
     misc_feature    207..221
                     /label=BBa_B0030
     RBS             207..221
                     /label=BBa_B0030
     CDS             228..1418
                     /label=tetA(C) forward
     primer_bind     228..251
                     /label=Fwd primer J31007
     primer_bind     1399..1418
                     /label=Rev primer J31007
     misc_feature    1416..1418
                     /label=stop
     CDS             228..1418
                     /label=BBa_J31007
     misc_feature    1427..1452
                     /label=BBa_J44000
     stem_loop       1472..1515
                     /label=stem_loop
     misc_feature    1461..1540
                     /label=BBa_B0010
     terminator      1461..1540
                     /label=BBa_B0010
     stem_loop       1556..1575
                     /label=T7 TE
     misc_feature    1582..1582
                     /label=stop
     polyA_site      1576..1589
                     /label=polya
     misc_feature    1549..1589
                     /label=BBa_B0012
     terminator      1549..1589
                     /label=BBa_B0012
     misc_feature    1648..1653
                     /label=Former SpeI site
     misc_feature    1648..1648
                     /label=Mutation of SpeI site
     misc_feature    1598..1674
                     /label=RE Sequence
     misc_feature    1626..1626
                     /label=Originally a C
     misc_feature    1674..1674
                     /label=Insertion right before biobrick ends
     misc_feature    1603..1618
                     /label=Proximal Fis Binding Site
     misc_feature    1652..1666
                     /label=Distal Fis Binding Site
     misc_feature    1598..1674
                     /label=BBa_J3101
     RBS             1683..1697
                     /label=RBS-1\Strong
     RBS             1690..1694
                     /label=RBS
     misc_feature    1683..1697
                     /label=BBa_B0030
     RBS             1683..1697
                     /label=BBa_B0030
     CDS             1704..2894
                     /label=tetA(C) forward
     primer_bind     1704..1727
                     /label=Fwd primer J31007
     primer_bind     2875..2894
                     /label=Rev primer J31007
     misc_feature    2892..2894
                     /label=stop
     CDS             1704..2894
                     /label=BBa_J31007
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagacattg attatttgca cggcgtcaca
       61 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
      121 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agttatcaaa
      181 aaccatggtt tttgataata ctagagatta aagaggagaa atactagatg aaatctaaca
      241 atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
      301 tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact
      361 atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag
      421 cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta
      481 tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg
      541 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
      601 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
      661 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
      721 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
      781 ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
      841 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
      901 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
      961 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     1021 tcgtcactgg ccccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     1081 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     1141 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     1201 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     1261 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     1321 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     1381 tgcgtcgcgg tgcatggagc cgggccacct cgacctaata ctagagttat caaaaaccat
     1441 ggtttttgat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1501 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1561 accttcgggt gggcctttct gcgtttatat actagagttc gggtgtcaac aattgaccaa
     1621 aatattgatt tacagcgtaa tgcgctttct agtgcaaatt gtgaccgcat tttgtactag
     1681 agattaaaga ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca
     1741 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc
     1801 gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg
     1861 cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc
     1921 gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca
     1981 cacccgtcct gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     2041 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     2101 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     2161 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     2221 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc
     2281 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     2341 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     2401 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     2461 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac
     2521 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     2581 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     2641 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     2701 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
     2761 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     2821 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     2881 ccacctcgac ctaa
//