LOCUS       BBa_S03672              1456 bp    DNA     linear       19-Nov-2024
DEFINITION  pLac-HixC : TetB-RBS<sub>rev</sub>
ACCESSION   BBa_S03672
VERSION     BBa_S03672.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..234
                     /label=BBa_J44000
     CDS             243..1433
                     /label=tetA(C) backwards
     misc_feature    243..245
                     /label=stop
     primer_bind     243..262
                     /label=Rev primer J31006
     primer_bind     1410..1433
                     /label=Fwd primer J31006
     CDS             243..1433
                     /label=BBa_J31006
     RBS             1442..1456
                     /label=Reverse RBS
     RBS             1442..1456
                     /label=BBa_J44001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtt atcaaaaacc atggtttttg ataatactag
      241 agttaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
      301 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
      361 atcgccgtga cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat
      421 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
      481 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
      541 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
      601 tgcttctcgc cgaaacgttt ggtggcgggg ccagtgacga aggcttgagc gagggcgtgc
      661 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
      721 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
      781 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
      841 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag
      901 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
      961 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     1021 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     1081 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     1141 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     1201 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     1261 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     1321 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     1381 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt cattactaga
     1441 gtttctcctc tttaat
//