LOCUS       BBa_S03673              2199 bp    DNA     linear       19-Nov-2024
DEFINITION  (1)-2 RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC : 
            pLac-HixC-TetB-RBS<sub>rev</sub>
ACCESSION   BBa_S03673
VERSION     BBa_S03673.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     CDS             744..831
                     /label=end of LacI coding region (inactive)
     misc_feature    744..943
                     /label=BBa_R0010
     misc_feature    832..869
                     /label=CAP binding site
     promoter        880..885
                     /label=-35
     promoter        904..909
                     /label=-10
     misc_feature    909..943
                     /label=LacI binding site
     misc_feature    916..916
                     /label=start
     promoter        744..943
                     /label=BBa_R0010
     misc_feature    952..977
                     /label=BBa_J44000
     CDS             986..2176
                     /label=tetA(C) backwards
     misc_feature    986..988
                     /label=stop
     primer_bind     986..1005
                     /label=Rev primer J31006
     primer_bind     2153..2176
                     /label=Fwd primer J31006
     CDS             986..2176
                     /label=BBa_J31006
     RBS             2185..2199
                     /label=Reverse RBS
     RBS             2185..2199
                     /label=BBa_J44001
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat
      781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gttatcaaaa
      961 accatggttt ttgataatac tagagttagg tcgaggtggc ccggctccat gcaccgcgac
     1021 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     1081 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta
     1141 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     1201 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     1261 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     1321 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg gggccagtga
     1381 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     1441 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     1501 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     1561 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
     1621 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     1681 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     1741 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     1801 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     1861 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
     1921 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
     1981 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
     2041 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
     2101 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
     2161 cattgttaga tttcattact agagtttctc ctctttaat
//