LOCUS       BBa_S03674              1452 bp    DNA     linear       20-Dec-2024
DEFINITION  (-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
ACCESSION   BBa_S03674
VERSION     BBa_S03674.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     promoter        35..164
                     /label=promoter
     promoter        35..164
                     /label=BBa_J44002
     misc_feature    173..198
                     /label=BBa_J44000
     RBS             207..221
                     /label=RBS-1\Strong
     RBS             214..218
                     /label=RBS
     misc_feature    207..221
                     /label=BBa_B0030
     RBS             207..221
                     /label=BBa_B0030
     CDS             228..1418
                     /label=tetA(C) forward
     primer_bind     228..251
                     /label=Fwd primer J31007
     primer_bind     1399..1418
                     /label=Rev primer J31007
     misc_feature    1416..1418
                     /label=stop
     CDS             228..1418
                     /label=BBa_J31007
     misc_feature    1427..1452
                     /label=BBa_J44000
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agaggctagc ccaaaaaaac ggtatggaga
       61 aacagtagag agttgcgata aaaagcgtca ggtaggatcc gctaatctta tggataaaaa
      121 tgctatggca tagcaaagtg tgacgccgtg caaataatca atgttactag agttatcaaa
      181 aaccatggtt tttgataata ctagagatta aagaggagaa atactagatg aaatctaaca
      241 atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
      301 tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact
      361 atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag
      421 cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta
      481 tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg
      541 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
      601 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
      661 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
      721 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
      781 ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
      841 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
      901 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
      961 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     1021 tcgtcactgg ccccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     1081 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     1141 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     1201 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     1261 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     1321 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     1381 tgcgtcgcgg tgcatggagc cgggccacct cgacctaata ctagagttat caaaaaccat
     1441 ggtttttgat aa
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