##gff-version 3
##sequence-region BBa_S03677 1 2231
BBa_S03677	.	promoter	1998	2197	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03677	.	non_covalent_binding_site	2086	2123	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03677	.	engineered_region	1998	2197	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03677	.	promoter	2158	2163	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03677	.	start_codon	2170	2170	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03677	.	non_covalent_binding_site	2163	2197	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03677	.	CDS	1998	2085	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03677	.	promoter	2134	2139	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03677	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_S03677	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_S03677	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_S03677	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_S03677	.	sequence_feature	710	735	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03677	.	CDS	765	1955	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03677	.	CDS	765	1955	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03677	.	primer_binding_site	1936	1955	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03677	.	primer_binding_site	765	788	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03677	.	stop_codon	1953	1955	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03677	.	sequence_feature	1964	1989	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03677	.	sequence_feature	2206	2231	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03677	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03677	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03677	.	ribosome_entry_site	744	758	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03677	.	ribosome_entry_site	751	755	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03677	.	engineered_region	744	758	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03677	.	ribosome_entry_site	744	758	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_S03677
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagtt
atcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgt
catcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatc
gtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccg
ttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgc
gatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcg
gttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcg
gcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggc
ggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgccc
ttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttct
ttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgac
gatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaa
cgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgc
gaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtc
caggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattgga
ccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctat
accttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaacc
atggtttttgataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggc
tttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagttatcaa
aaaccatggtttttgataa