BBa_S03679
type: ComponentDefinition
persistent identity: BBa S03679
display id: BBa_S03679
version: 1
was generated by: iGEM to SBOL conversion
description: (2,-1) RFPrev-RBSrev-HixC-RBS-TetF-HixC : pLacrev-HixC
created: 2007-05-07T11:00:00Z
modified: 2015-05-08T01:14:28Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J44000
component: BBa_J31007
component: BBa_J31013
component: BBa_J44001
component: BBa_J31008
component: BBa_B0030
sequence annotation: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_J44000
sequence annotation: BBa_B0030
sequence annotation: BBa_J31007
sequence annotation: BBa_J31013
sequence: https://synbiohub.org/public/igem/BBa_S03679_sequence/1

BBa_J44000
type: Component
persistent identity: component1932433
display id: component1932433
version: 1
top level: BBa_S03679
definition: hixC
access: public

BBa_J31007
type: Component
persistent identity: component1932432
display id: component1932432
version: 1
top level: BBa_S03679
definition: tetA(C)f
access: public

BBa_J44000
type: Component
persistent identity: component1932441
display id: component1932441
version: 1
top level: BBa_S03679
definition: hixC
access: public

BBa_J31013
type: Component
persistent identity: component1932440
display id: component1932440
version: 1
top level: BBa_S03679
definition: BBa_J31013
access: public

BBa_J44001
type: Component
persistent identity: component1932423
display id: component1932423
version: 1
top level: BBa_S03679
definition: BBa_J44001
access: public

BBa_J31008
type: Component
persistent identity: component1932421
display id: component1932421
version: 1
top level: BBa_S03679
definition: mRFPr
access: public

BBa_B0030
type: Component
persistent identity: component1932426
display id: component1932426
version: 1
top level: BBa_S03679
definition: BBa_B0030
access: public

BBa_J44000
type: Component
persistent identity: component1932424
display id: component1932424
version: 1
top level: BBa_S03679
definition: hixC
access: public

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation1932421
display id: annotation1932421
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932421/range1932421/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_S03679/annotation1932421/range1932421/1
type: Range
persistent identity: range1932421
display id: range1932421
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 1
end: 678
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation1932423
display id: annotation1932423
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932423/range1932423/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_S03679/annotation1932423/range1932423/1
type: Range
persistent identity: range1932423
display id: range1932423
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 687
end: 701
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932424
display id: annotation1932424
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932424/range1932424/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03679/annotation1932424/range1932424/1
type: Range
persistent identity: range1932424
display id: range1932424
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 710
end: 735
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1932426
display id: annotation1932426
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932426/range1932426/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03679/annotation1932426/range1932426/1
type: Range
persistent identity: range1932426
display id: range1932426
version: 1
top level: BBa_S03679
direction: direction/forward
start: 744
end: 758
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1932432
display id: annotation1932432
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932432/range1932432/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_S03679/annotation1932432/range1932432/1
type: Range
persistent identity: range1932432
display id: range1932432
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 765
end: 1955
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932433
display id: annotation1932433
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932433/range1932433/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03679/annotation1932433/range1932433/1
type: Range
persistent identity: range1932433
display id: range1932433
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 1964
end: 1989
orientation: inline

BBa_J31013
type: SequenceAnnotation
persistent identity: annotation1932440
display id: annotation1932440
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932440/range1932440/1
component: BBa_J31013

https://synbiohub.org/public/igem/BBa_S03679/annotation1932440/range1932440/1
type: Range
persistent identity: range1932440
display id: range1932440
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 1998
end: 2197
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932441
display id: annotation1932441
version: 1
top level: BBa_S03679
location: https://synbiohub.org/public/igem/BBa_S03679/annotation1932441/range1932441/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03679/annotation1932441/range1932441/1
type: Range
persistent identity: range1932441
display id: range1932441
version: 1
top level: BBa_S03679
direction: direction/reverse
start: 2206
end: 2231
orientation: inline

BBa_J31013
type: ComponentDefinition
persistent identity: BBa J31013
display id: BBa_J31013
version: 1
was generated by: iGEM to SBOL conversion
description: pLac Backwards [cf. BBa_R0010]
created: 2007-04-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: BBa_R0010
owned by: james
owned by: myers
mutable description: Promoter from the lack operon cloned in the reverse orientation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Promoter from the lack operon cloned in the reverse orientation.
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: R0010 reverse
sequence annotation: LacI binding site
sequence annotation: -10
sequence annotation: -35
sequence annotation: CAP binding site
sequence annotation: end of LacI coding region (inactive)
sequence: https://synbiohub.org/public/igem/BBa_J31013_sequence/1

R0010 reverse
type: SequenceAnnotation
persistent identity: annotation1928668
display id: annotation1928668
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1
type: Range
persistent identity: range1928668
display id: range1928668
version: 1
top level: BBa_J31013
direction: direction/reverse
start: 1
end: 200
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1928673
display id: annotation1928673
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1
type: Range
persistent identity: range1928673
display id: range1928673
version: 1
top level: BBa_J31013
direction: direction/forward
start: 1
end: 34
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1928672
display id: annotation1928672
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1
type: Range
persistent identity: range1928672
display id: range1928672
version: 1
top level: BBa_J31013
direction: direction/reverse
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1928671
display id: annotation1928671
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1
type: Range
persistent identity: range1928671
display id: range1928671
version: 1
top level: BBa_J31013
direction: direction/reverse
start: 58
end: 64
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1928670
display id: annotation1928670
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1
type: Range
persistent identity: range1928670
display id: range1928670
version: 1
top level: BBa_J31013
direction: direction/forward
start: 74
end: 112
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1928669
display id: annotation1928669
version: 1
top level: BBa_J31013
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1
type: Range
persistent identity: range1928669
display id: range1928669
version: 1
top level: BBa_J31013
direction: direction/reverse
start: 113
end: 200
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP reverse
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mRFP reverse
sequence annotation: Xba mRFP R
sequence annotation: Spe mRFP F
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
top level: mRFPr
direction: direction/reverse
start: 1
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
top level: mRFPr
direction: direction/forward
start: 1
end: 20
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
top level: mRFPr
direction: direction/reverse
start: 659
end: 678
orientation: inline

https://synbiohub.org/public/igem/BBa_S03679_sequence/1
type: Sequence
persistent identity: BBa S03679 sequence
display id: BBa_S03679_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagttatcaaaaaccatggtttttgataatactagagtgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31013_sequence/1
type: Sequence
persistent identity: BBa J31013 sequence
display id: BBa_J31013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31008_sequence/1
type: Sequence
persistent identity: BBa J31008 sequence
display id: BBa_J31008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z