##gff-version 3
##sequence-region BBa_S03680 1 2233
BBa_S03680	.	CDS	744	1934	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_S03680	.	CDS	744	1934	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03680	.	primer_binding_site	744	763	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S03680	.	primer_binding_site	1911	1934	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S03680	.	stop_codon	744	746	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03680	.	ribosome_entry_site	1943	1957	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03680	.	ribosome_entry_site	1943	1957	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03680	.	promoter	2000	2199	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03680	.	promoter	2160	2165	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03680	.	start_codon	2172	2172	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03680	.	CDS	2000	2087	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03680	.	non_covalent_binding_site	2088	2125	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03680	.	non_covalent_binding_site	2165	2199	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03680	.	promoter	2136	2141	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03680	.	engineered_region	2000	2199	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03680	.	sequence_feature	1966	1991	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03680	.	sequence_feature	710	735	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03680	.	sequence_feature	2208	2233	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03680	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_S03680	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_S03680	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_S03680	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_S03680	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03680	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
>BBa_S03680
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagtt
atcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcgggga
ggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgt
gacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtca
tctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaagg
ccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgctt
ctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac
aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacga
gttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggtt
gaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgagg
ccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca
ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatata
ggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtg
gtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggaca
gtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgct
gtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacg
gtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaa
ccatggtttttgataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccag
gctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagttatc
aaaaaccatggtttttgataa