##gff-version 3
##sequence-region BBa_S03681 1 2233
BBa_S03681	.	promoter	2000	2199	.	+	0	ID=BBa_J31013;Name=BBa_J31013
BBa_S03681	.	non_covalent_binding_site	2000	2033	.	+	0	ID=annotation1928673;Name=LacI binding site;Parent=BBa_J31013
BBa_S03681	.	promoter	2057	2063	.	+	0	ID=annotation1928671;Name=-35;Parent=BBa_J31013
BBa_S03681	.	engineered_region	2000	2199	.	+	0	ID=annotation1928668;Name=R0010 reverse;Parent=BBa_J31013
BBa_S03681	.	promoter	2034	2039	.	+	0	ID=annotation1928672;Name=-10;Parent=BBa_J31013
BBa_S03681	.	CDS	2112	2199	.	+	0	ID=annotation1928669;Name=end of LacI coding region (inactive);Parent=BBa_J31013
BBa_S03681	.	non_covalent_binding_site	2073	2111	.	+	0	ID=annotation1928670;Name=CAP binding site;Parent=BBa_J31013
BBa_S03681	.	sequence_feature	1966	1991	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03681	.	sequence_feature	2208	2233	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03681	.	CDS	744	1934	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_S03681	.	stop_codon	744	746	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03681	.	primer_binding_site	744	763	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S03681	.	CDS	744	1934	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03681	.	primer_binding_site	1911	1934	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S03681	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03681	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03681	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_S03681	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_S03681	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_S03681	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_S03681	.	sequence_feature	710	735	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03681	.	ribosome_entry_site	1943	1957	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03681	.	ribosome_entry_site	1943	1957	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
>BBa_S03681
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagtt
atcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcgggga
ggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgt
gacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtca
tctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaagg
ccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgctt
ctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac
aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacga
gttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggtt
gaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgagg
ccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca
ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatata
ggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtg
gtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggaca
gtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgct
gtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacg
gtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaa
ccatggtttttgataatactagagtgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcata
aagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgg
gaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgtactagagttatc
aaaaaccatggtttttgataa