LOCUS       BBa_S03683               977 bp    DNA     linear       20-Dec-2024
DEFINITION  RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC : pLac-HixC
ACCESSION   BBa_S03683
VERSION     BBa_S03683.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     CDS             744..831
                     /label=end of LacI coding region (inactive)
     misc_feature    744..943
                     /label=BBa_R0010
     misc_feature    832..869
                     /label=CAP binding site
     promoter        880..885
                     /label=-35
     promoter        904..909
                     /label=-10
     misc_feature    909..943
                     /label=LacI binding site
     misc_feature    916..916
                     /label=start
     promoter        744..943
                     /label=BBa_R0010
     misc_feature    952..977
                     /label=BBa_J44000
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat
      781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gttatcaaaa
      961 accatggttt ttgataa
//