LOCUS       BBa_S03684              2231 bp    DNA     linear       20-Dec-2024
DEFINITION  (1,2) RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-pLac-HixC : 
            RBS-TetF-HixC
ACCESSION   BBa_S03684
VERSION     BBa_S03684.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     CDS             744..831
                     /label=end of LacI coding region (inactive)
     misc_feature    744..943
                     /label=BBa_R0010
     misc_feature    832..869
                     /label=CAP binding site
     promoter        880..885
                     /label=-35
     promoter        904..909
                     /label=-10
     misc_feature    909..943
                     /label=LacI binding site
     misc_feature    916..916
                     /label=start
     promoter        744..943
                     /label=BBa_R0010
     misc_feature    952..977
                     /label=BBa_J44000
     RBS             986..1000
                     /label=RBS-1\Strong
     RBS             993..997
                     /label=RBS
     misc_feature    986..1000
                     /label=BBa_B0030
     RBS             986..1000
                     /label=BBa_B0030
     CDS             1007..2197
                     /label=tetA(C) forward
     primer_bind     1007..1030
                     /label=Fwd primer J31007
     primer_bind     2178..2197
                     /label=Rev primer J31007
     misc_feature    2195..2197
                     /label=stop
     CDS             1007..2197
                     /label=BBa_J31007
     misc_feature    2206..2231
                     /label=BBa_J44000
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat
      781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gttatcaaaa
      961 accatggttt ttgataatac tagagattaa agaggagaaa tactagatga aatctaacaa
     1021 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
     1081 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
     1141 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
     1201 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
     1261 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
     1321 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     1381 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     1441 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     1501 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     1561 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     1621 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     1681 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     1741 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     1801 cgtcactggc cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     1861 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     1921 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     1981 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     2041 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     2101 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     2161 gcgtcgcggt gcatggagcc gggccacctc gacctaatac tagagttatc aaaaaccatg
     2221 gtttttgata a
//