BBa_S03684
type: ComponentDefinition
persistent identity: BBa S03684
display id: BBa_S03684
version: 1
was generated by: iGEM to SBOL conversion
description: (1,2) RFPrev-RBSrev-HixC-pLac-HixC : RBS-TetF-HixC
created: 2007-05-07T11:00:00Z
modified: 2015-05-08T01:14:28Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J44000
component: BBa_J31007
component: BBa_B0030
component: BBa_R0010
component: BBa_J44001
component: BBa_J31008
sequence annotation: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_J44000
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_S03684_sequence/1

BBa_J44000
type: Component
persistent identity: component1932550
display id: component1932550
version: 1
top level: BBa_S03684
definition: hixC
access: public

BBa_J31007
type: Component
persistent identity: component1932549
display id: component1932549
version: 1
top level: BBa_S03684
definition: tetA(C)f
access: public

BBa_B0030
type: Component
persistent identity: component1932543
display id: component1932543
version: 1
top level: BBa_S03684
definition: BBa_B0030
access: public

BBa_J44000
type: Component
persistent identity: component1932541
display id: component1932541
version: 1
top level: BBa_S03684
definition: hixC
access: public

BBa_R0010
type: Component
persistent identity: component1932534
display id: component1932534
version: 1
top level: BBa_S03684
definition: LacI
access: public

BBa_J44000
type: Component
persistent identity: component1932533
display id: component1932533
version: 1
top level: BBa_S03684
definition: hixC
access: public

BBa_J44001
type: Component
persistent identity: component1932532
display id: component1932532
version: 1
top level: BBa_S03684
definition: BBa_J44001
access: public

BBa_J31008
type: Component
persistent identity: component1932530
display id: component1932530
version: 1
top level: BBa_S03684
definition: mRFPr
access: public

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation1932530
display id: annotation1932530
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932530/range1932530/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_S03684/annotation1932530/range1932530/1
type: Range
persistent identity: range1932530
display id: range1932530
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 1
end: 678
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation1932532
display id: annotation1932532
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932532/range1932532/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_S03684/annotation1932532/range1932532/1
type: Range
persistent identity: range1932532
display id: range1932532
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 687
end: 701
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932533
display id: annotation1932533
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932533/range1932533/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03684/annotation1932533/range1932533/1
type: Range
persistent identity: range1932533
display id: range1932533
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 710
end: 735
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1932534
display id: annotation1932534
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932534/range1932534/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S03684/annotation1932534/range1932534/1
type: Range
persistent identity: range1932534
display id: range1932534
version: 1
top level: BBa_S03684
direction: direction/forward
start: 744
end: 943
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932541
display id: annotation1932541
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932541/range1932541/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03684/annotation1932541/range1932541/1
type: Range
persistent identity: range1932541
display id: range1932541
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 952
end: 977
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1932543
display id: annotation1932543
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932543/range1932543/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03684/annotation1932543/range1932543/1
type: Range
persistent identity: range1932543
display id: range1932543
version: 1
top level: BBa_S03684
direction: direction/forward
start: 986
end: 1000
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1932549
display id: annotation1932549
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932549/range1932549/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_S03684/annotation1932549/range1932549/1
type: Range
persistent identity: range1932549
display id: range1932549
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 1007
end: 2197
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1932550
display id: annotation1932550
version: 1
top level: BBa_S03684
location: https://synbiohub.org/public/igem/BBa_S03684/annotation1932550/range1932550/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_S03684/annotation1932550/range1932550/1
type: Range
persistent identity: range1932550
display id: range1932550
version: 1
top level: BBa_S03684
direction: direction/reverse
start: 2206
end: 2231
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP reverse
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mRFP reverse
sequence annotation: Xba mRFP R
sequence annotation: Spe mRFP F
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
top level: mRFPr
direction: direction/reverse
start: 1
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
top level: mRFPr
direction: direction/forward
start: 1
end: 20
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
top level: mRFPr
direction: direction/reverse
start: 659
end: 678
orientation: inline

https://synbiohub.org/public/igem/BBa_S03684_sequence/1
type: Sequence
persistent identity: BBa S03684 sequence
display id: BBa_S03684_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagttatcaaaaaccatggtttttgataatactagagattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31008_sequence/1
type: Sequence
persistent identity: BBa J31008 sequence
display id: BBa_J31008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z