##gff-version 3
##sequence-region BBa_S03685 1 2233
BBa_S03685	.	sequence_feature	710	735	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03685	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_S03685	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_S03685	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_S03685	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_S03685	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03685	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03685	.	sequence_feature	952	977	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_S03685	.	ribosome_entry_site	2185	2199	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03685	.	ribosome_entry_site	2185	2199	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03685	.	promoter	744	943	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03685	.	engineered_region	744	943	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03685	.	CDS	744	831	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03685	.	non_covalent_binding_site	909	943	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03685	.	non_covalent_binding_site	832	869	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03685	.	promoter	904	909	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03685	.	promoter	880	885	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03685	.	start_codon	916	916	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03685	.	CDS	986	2176	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_S03685	.	CDS	986	2176	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S03685	.	primer_binding_site	986	1005	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S03685	.	stop_codon	986	988	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S03685	.	primer_binding_site	2153	2176	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S03685	.	sequence_feature	2208	2233	.	+	0	ID=BBa_J44000;Name=BBa_J44000
>BBa_S03685
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagtt
atcaaaaaccatggtttttgataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattagg
caccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatacta
gagttatcaaaaaccatggtttttgataatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgc
ggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatc
gccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggt
cgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggg
gaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcc
tgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaa
gcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcc
tacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgact
gggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggt
tgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcc
tgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcg
atataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgg
gtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtc
ggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcg
atgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccaggg
tgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatc
aaaaaccatggtttttgataa