LOCUS BBa_S03686 977 bp DNA linear 22-Nov-2024
DEFINITION RFPrevRBSrev-HixC : pLacrev-HixC
(dummy part)
ACCESSION BBa_S03686
VERSION BBa_S03686.1
FEATURES Location/Qualifiers
CDS 1..678
/label=mRFP reverse
primer_bind 659..678
/label=Spe mRFP F
primer_bind 1..20
/label=Xba mRFP R
misc_feature 1..678
/label=BBa_J31008
RBS 687..701
/label=Reverse RBS
RBS 687..701
/label=BBa_J44001
misc_feature 710..735
/label=BBa_J44000
misc_feature 744..943
/label=R0010 reverse
CDS 856..943
/label=end of LacI coding region (inactive)
misc_feature 817..855
/label=CAP binding site
promoter 801..807
/label=-35
promoter 778..783
/label=-10
misc_feature 744..777
/label=LacI binding site
promoter 744..943
/label=BBa_J31013
misc_feature 952..977
/label=BBa_J44000
ORIGIN
1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
721 catggttttt gataatacta gagtgtgtga aattgttatc cgctcacaat tccacacaac
781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgtactaga gttatcaaaa
961 accatggttt ttgataa
//