LOCUS BBa_S03686 977 bp DNA linear 22-Nov-2024 DEFINITION RFPrevRBSrev-HixC : pLacrev-HixC (dummy part) ACCESSION BBa_S03686 VERSION BBa_S03686.1 FEATURES Location/Qualifiers CDS 1..678 /label=mRFP reverse primer_bind 659..678 /label=Spe mRFP F primer_bind 1..20 /label=Xba mRFP R misc_feature 1..678 /label=BBa_J31008 RBS 687..701 /label=Reverse RBS RBS 687..701 /label=BBa_J44001 misc_feature 710..735 /label=BBa_J44000 misc_feature 744..943 /label=R0010 reverse CDS 856..943 /label=end of LacI coding region (inactive) misc_feature 817..855 /label=CAP binding site promoter 801..807 /label=-35 promoter 778..783 /label=-10 misc_feature 744..777 /label=LacI binding site promoter 744..943 /label=BBa_J31013 misc_feature 952..977 /label=BBa_J44000 ORIGIN 1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc 61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac 121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag 181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt 241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt 301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac 361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa 421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta 481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt 541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa 601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac 661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac 721 catggttttt gataatacta gagtgtgtga aattgttatc cgctcacaat tccacacaac 781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgtactaga gttatcaaaa 961 accatggttt ttgataa //