RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Built by Josh as an intermediate in screening plasmid construction.
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
kanamycin resistance forward (KanF) [cf. BBa_J23012 & BBa_J31002]
If there is a promoter and an RBS before this region, the cell will express Kanamycin resistance. As it has full biobricks, you can easily add KanR to an existing part without changing the plasmid vector,
Conversion of the iGEM parts registry to SBOL2.1