LOCUS       BBa_S03723              2783 bp    DNA     linear       16-Feb-2025
DEFINITION  RBS-Kan-RBS-Tet-RBS-RFP (pSB1A2)
ACCESSION   BBa_S03723
VERSION     BBa_S03723.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..834
                     /label=KanR Gene
     CDS             19..834
                     /label=BBa_J31003
     misc_feature    1..834
                     /label=BBa_S03709
     misc_feature    847..850
                     /label=conserved
     RBS             843..854
                     /label=BBa_B0034
     CDS             861..2051
                     /label=tetA(C) forward
     primer_bind     861..884
                     /label=Fwd primer J31007
     primer_bind     2032..2051
                     /label=Rev primer J31007
     misc_feature    2049..2051
                     /label=stop
     CDS             861..2051
                     /label=BBa_J31007
     misc_feature    843..2051
                     /label=BBa_S03710
     misc_feature    2064..2067
                     /label=conserved
     RBS             2060..2071
                     /label=BBa_B0034
     CDS             2078..2752
                     /label=mrfp1
     misc_feature    2759..2783
                     /label=Help:Barcodes
     CDS             2078..2758
                     /label=BBa_E1010
     misc_feature    2060..2783
                     /label=BBa_I13502
     misc_feature    843..2783
                     /label=BBa_S03720
ORIGIN
        1 aaagaggaga aatactagat gagccatatt caacgggaaa cgtcttgctc caggccgcga
       61 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      121 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
      181 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      241 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      301 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
      361 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      421 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
      481 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
      541 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
      601 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
      661 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
      721 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
      781 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatactag
      841 agaaagagga gaaatactag atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
      901 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
      961 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1021 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1081 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1141 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     1201 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     1261 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     1321 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     1381 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
     1441 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     1501 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     1561 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     1621 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt
     1681 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     1741 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     1801 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     1861 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga
     1921 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
     1981 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
     2041 cctcgaccta atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
     2101 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     2161 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     2221 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     2281 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     2341 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     2401 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     2461 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     2521 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     2581 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     2641 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     2701 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     2761 ctgatagtgc tagtgtagat cgc
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