##gff-version 3
##sequence-region BBa_S03729 1 2074
BBa_S03729	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_S03729	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_S03729	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_S03729	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_S03729	.	terminator	775	815	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03729	.	stem_loop	782	801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03729	.	stop_codon	808	808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03729	.	engineered_region	775	815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03729	.	polyA_site	802	815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03729	.	CDS	884	2074	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S03729	.	CDS	884	2074	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S03729	.	primer_binding_site	2055	2074	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S03729	.	primer_binding_site	884	907	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S03729	.	stop_codon	2072	2074	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S03729	.	terminator	687	766	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03729	.	stem_loop	698	741	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03729	.	engineered_region	687	766	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03729	.	ribosome_entry_site	866	877	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03729	.	conserved_region	870	873	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03729	.	sequence_feature	824	857	.	+	0	ID=BBa_J61046;Name=BBa_J61046
>BBa_S03729
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagataacttcgtataatgtatgctatacgaagttattactagagaaag
aggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcatagg
cttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctg
ctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccag
tcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgc
cggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaa
gatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgt
tgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcct
aatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcg
cggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctct
gggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgca
cgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcg
gccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgctt
ccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaagg
atcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagc
acatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatgga
gccgggccacctcgacctaa