LOCUS       BBa_S03730              1001 bp    DNA     linear       25-Nov-2024
DEFINITION  Hin-LVA with inducible promoter
ACCESSION   BBa_S03730
VERSION     BBa_S03730.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=Reverse RBS
     RBS             1..15
                     /label=BBa_J44001
     CDS             24..111
                     /label=end of LacI coding region (inactive)
     misc_feature    24..223
                     /label=BBa_R0010
     misc_feature    112..149
                     /label=CAP binding site
     promoter        160..165
                     /label=-35
     promoter        184..189
                     /label=-10
     misc_feature    189..223
                     /label=LacI binding site
     misc_feature    196..196
                     /label=start
     promoter        24..223
                     /label=BBa_R0010
     RBS             232..246
                     /label=RBS-1\Strong
     RBS             239..243
                     /label=RBS
     misc_feature    232..246
                     /label=BBa_B0030
     RBS             232..246
                     /label=BBa_B0030
     CDS             253..822
                     /label=Hin
     primer_bind     253..255
                     /label=Fwd primer J31001
     misc_feature    823..861
                     /label=LVA
     misc_feature    862..864
                     /label=Stop codon
     primer_bind     803..864
                     /label=Rev primer J31001
     CDS             253..864
                     /label=BBa_J31001
     stem_loop       884..927
                     /label=stem_loop
     misc_feature    873..952
                     /label=BBa_B0010
     terminator      873..952
                     /label=BBa_B0010
     stem_loop       968..987
                     /label=T7 TE
     misc_feature    994..994
                     /label=stop
     polyA_site      988..1001
                     /label=polya
     misc_feature    961..1001
                     /label=BBa_B0012
     terminator      961..1001
                     /label=BBa_B0012
ORIGIN
        1 tttctcctct ttaattacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      121 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      181 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gattaaagag
      241 gagaaatact agatggctac tattgggtat attcgggtgt caacaattga ccaaaatatc
      301 gatttacagc gtaatgcgct taccagtgca aattgtgacc gcatttttga ggaccgtatc
      361 agtggcaaga ttgcaaaccg ccccggcctg aaacgagcgt taaagtatgt aaataaaggc
      421 gatactcttg tcgtctggaa attagacaga ctgggccgca gcgtgaaaaa cctggtggcg
      481 ttaatatcag aattacatga acgtggagct cacttccatt ctttaaccga tagtattgat
      541 accagtagcg cgatggggcg attctttttt catgtaatgt cagcactggc cgagatggag
      601 cgagaattaa ttgtcgagcg aacccttgcc ggactggctg ccgccagagc gcaaggacga
      661 ctgggagggc gccctcgggc gatcaacaga catgaacagg aacagattag tcggctatta
      721 gagaaaggcc atcctcggca gcaactagct attatttttg gtattggcgt atctacctta
      781 tacagatatt ttccggcaag ccgtataaaa aaacgaatga ataggcctgc tgcaaacgac
      841 gaaaactacg ctttagtagc ttaatactag agccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//