##gff-version 3
##sequence-region BBa_S03730 1 1001
BBa_S03730	.	ribosome_entry_site	232	246	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S03730	.	engineered_region	232	246	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03730	.	ribosome_entry_site	239	243	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03730	.	ribosome_entry_site	232	246	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03730	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_S03730	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_S03730	.	terminator	961	1001	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03730	.	stem_loop	968	987	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03730	.	polyA_site	988	1001	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03730	.	stop_codon	994	994	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03730	.	engineered_region	961	1001	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03730	.	CDS	253	864	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_S03730	.	stop_codon	862	864	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_S03730	.	primer_binding_site	253	255	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_S03730	.	primer_binding_site	803	864	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_S03730	.	CDS	253	822	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_S03730	.	tag	823	861	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_S03730	.	promoter	24	223	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S03730	.	CDS	24	111	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03730	.	non_covalent_binding_site	189	223	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03730	.	promoter	184	189	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03730	.	promoter	160	165	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03730	.	non_covalent_binding_site	112	149	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03730	.	start_codon	196	196	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03730	.	engineered_region	24	223	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03730	.	terminator	873	952	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03730	.	stem_loop	884	927	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03730	.	engineered_region	873	952	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S03730
tttctcctctttaattactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcac
gacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccagg
ctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaa
gaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatg
cgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacg
agcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctg
gtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcga
tggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccgg
actggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagt
cggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatatt
ttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata