BBa_S03730
type: ComponentDefinition
persistent identity: BBa S03730
display id: BBa_S03730
version: 1
was generated by: iGEM to SBOL conversion
description: Hin-LVA with inducible promoter
created: 2007-07-02T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1518
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Jordan Baumgardner
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_R0010
component: BBa_J44001
component: BBa_B0012
component: BBa_B0010
component: BBa_J31001
component: BBa_B0030
sequence annotation: BBa_J44001
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03730_sequence/1

BBa_R0010
type: Component
persistent identity: component1936206
display id: component1936206
version: 1
top level: BBa_S03730
definition: LacI
access: public

BBa_J44001
type: Component
persistent identity: component1936205
display id: component1936205
version: 1
top level: BBa_S03730
definition: BBa_J44001
access: public

BBa_B0012
type: Component
persistent identity: component1936224
display id: component1936224
version: 1
top level: BBa_S03730
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1936222
display id: component1936222
version: 1
top level: BBa_S03730
definition: BBa_B0010
access: public

BBa_J31001
type: Component
persistent identity: component1936221
display id: component1936221
version: 1
top level: BBa_S03730
definition: Hin LVA
access: public

BBa_B0030
type: Component
persistent identity: component1936214
display id: component1936214
version: 1
top level: BBa_S03730
definition: BBa_B0030
access: public

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation1936205
display id: annotation1936205
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936205/range1936205/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_S03730/annotation1936205/range1936205/1
type: Range
persistent identity: range1936205
display id: range1936205
version: 1
top level: BBa_S03730
direction: direction/reverse
start: 1
end: 15
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1936206
display id: annotation1936206
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936206/range1936206/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S03730/annotation1936206/range1936206/1
type: Range
persistent identity: range1936206
display id: range1936206
version: 1
top level: BBa_S03730
direction: direction/forward
start: 24
end: 223
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1936214
display id: annotation1936214
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936214/range1936214/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03730/annotation1936214/range1936214/1
type: Range
persistent identity: range1936214
display id: range1936214
version: 1
top level: BBa_S03730
direction: direction/forward
start: 232
end: 246
orientation: inline

BBa_J31001
type: SequenceAnnotation
persistent identity: annotation1936221
display id: annotation1936221
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936221/range1936221/1
component: BBa_J31001

https://synbiohub.org/public/igem/BBa_S03730/annotation1936221/range1936221/1
type: Range
persistent identity: range1936221
display id: range1936221
version: 1
top level: BBa_S03730
direction: direction/reverse
start: 253
end: 864
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1936222
display id: annotation1936222
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936222/range1936222/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03730/annotation1936222/range1936222/1
type: Range
persistent identity: range1936222
display id: range1936222
version: 1
top level: BBa_S03730
direction: direction/forward
start: 873
end: 952
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1936224
display id: annotation1936224
version: 1
top level: BBa_S03730
location: https://synbiohub.org/public/igem/BBa_S03730/annotation1936224/range1936224/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03730/annotation1936224/range1936224/1
type: Range
persistent identity: range1936224
display id: range1936224
version: 1
top level: BBa_S03730
direction: direction/forward
start: 961
end: 1001
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

Hin LVA
type: ComponentDefinition
persistent identity: BBa J31001
display id: BBa_J31001
version: 1
was generated by: iGEM to SBOL conversion
description: DNA invertase Hin tagged with LVA
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium and the the Hin part without LVA.
owned by: james
owned by: myers
mutable description: This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Fwd primer J31001
sequence annotation: Hin
sequence annotation: Rev primer J31001
sequence annotation: LVA
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_J31001_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910565
display id: annotation1910565
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
type: Range
persistent identity: range1910565
display id: range1910565
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin
type: SequenceAnnotation
persistent identity: annotation1910564
display id: annotation1910564
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
type: Range
persistent identity: range1910564
display id: range1910564
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 570
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910570
display id: annotation1910570
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
type: Range
persistent identity: range1910570
display id: range1910570
version: 1
top level: Hin LVA
direction: direction/reverse
start: 551
end: 612
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1910566
display id: annotation1910566
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
type: Range
persistent identity: range1910566
display id: range1910566
version: 1
top level: Hin LVA
direction: direction/forward
start: 571
end: 609
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1910567
display id: annotation1910567
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
type: Range
persistent identity: range1910567
display id: range1910567
version: 1
top level: Hin LVA
direction: direction/forward
start: 610
end: 612
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

https://synbiohub.org/public/igem/BBa_S03730_sequence/1
type: Sequence
persistent identity: BBa S03730 sequence
display id: BBa_S03730_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaattactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31001_sequence/1
type: Sequence
persistent identity: BBa J31001 sequence
display id: BBa_J31001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z