LOCUS       BBa_S03736              1459 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac-lox-RBS-Tet (in pSB1A2)
ACCESSION   BBa_S03736
VERSION     BBa_S03736.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..242
                     /label=BBa_J61046
     misc_feature    255..258
                     /label=conserved
     RBS             251..262
                     /label=BBa_B0034
     CDS             269..1459
                     /label=tetA(C) forward
     primer_bind     269..292
                     /label=Fwd primer J31007
     primer_bind     1440..1459
                     /label=Rev primer J31007
     misc_feature    1457..1459
                     /label=stop
     CDS             269..1459
                     /label=BBa_J31007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat aacttcgtat aatgtatgct atacgaagtt
      241 attactagag aaagaggaga aatactagat gaaatctaac aatgcgctca tcgtcatcct
      301 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      361 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      421 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      481 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      541 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      601 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      661 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      721 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      781 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      841 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      901 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      961 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     1021 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gccccgccac
     1081 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     1141 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1201 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1261 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     1321 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1381 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1441 ccgggccacc tcgacctaa
//