LOCUS       BBa_S03744              1731 bp    DNA     linear       14-May-2024
DEFINITION  S03706:P0440
ACCESSION   BBa_S03744
VERSION     BBa_S03744.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    896..899
                     /label=conserved
     RBS             892..903
                     /label=BBa_B0034
     CDS             913..1529
                     /label=tetR
     misc_feature    1530..1563
                     /label=SsrA
     misc_feature    1570..1594
                     /label=Help:Barcodes
     CDS             910..1569
                     /label=BBa_C0040
     stem_loop       1614..1657
                     /label=stem_loop
     misc_feature    1603..1682
                     /label=BBa_B0010
     terminator      1603..1682
                     /label=BBa_B0010
     stem_loop       1698..1717
                     /label=T7 TE
     misc_feature    1724..1724
                     /label=stop
     polyA_site      1718..1731
                     /label=polya
     misc_feature    1691..1731
                     /label=BBa_B0012
     terminator      1691..1731
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaaagaggag
      901 aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
      961 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
     1021 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
     1081 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
     1141 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
     1201 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
     1261 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
     1321 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
     1381 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
     1441 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
     1501 gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta
     1561 gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac
     1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1681 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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