##gff-version 3
##sequence-region BBa_S03744 1 1731
BBa_S03744	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03744	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03744	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03744	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03744	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03744	.	terminator	1691	1731	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03744	.	polyA_site	1718	1731	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03744	.	stem_loop	1698	1717	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03744	.	engineered_region	1691	1731	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03744	.	stop_codon	1724	1724	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03744	.	CDS	910	1569	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S03744	.	tag	1530	1563	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S03744	.	engineered_tag	1570	1594	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S03744	.	CDS	913	1529	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S03744	.	terminator	1603	1682	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S03744	.	stem_loop	1614	1657	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03744	.	engineered_region	1603	1682	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03744	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S03744	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S03744	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S03744	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S03744	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_S03744	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S03744	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03744	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03744	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03744	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03744	.	ribosome_entry_site	892	903	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S03744	.	conserved_region	896	899	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03744
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgca
ttagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagccta
cattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactca
cttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagt
catcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcct
ttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgt
attggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacga
caagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattag
aaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtg
ctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata