LOCUS       BBa_S03748              1541 bp    DNA     linear       22-Dec-2024
DEFINITION  tet-regulated N (Strong tetR promoter)
ACCESSION   BBa_S03748
VERSION     BBa_S03748.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    55..112
                     /label=spacer
     misc_feature    113..513
                     /label=N
     misc_feature    1..513
                     /label=BBa_I759001
     stem_loop       533..576
                     /label=stem_loop
     misc_feature    522..601
                     /label=BBa_B0010
     terminator      522..601
                     /label=BBa_B0010
     stem_loop       617..636
                     /label=T7 TE
     misc_feature    643..643
                     /label=stop
     polyA_site      637..650
                     /label=polya
     misc_feature    610..650
                     /label=BBa_B0012
     terminator      610..650
                     /label=BBa_B0012
     terminator      522..650
                     /label=BBa_B0015
     promoter        659..693
                     /label=BBa_J23100
     misc_feature    706..709
                     /label=conserved
     RBS             702..713
                     /label=BBa_B0034
     CDS             723..1339
                     /label=tetR
     misc_feature    1340..1373
                     /label=SsrA
     misc_feature    1380..1404
                     /label=Help:Barcodes
     CDS             720..1379
                     /label=BBa_C0040
     stem_loop       1424..1467
                     /label=stem_loop
     misc_feature    1413..1492
                     /label=BBa_B0010
     terminator      1413..1492
                     /label=BBa_B0010
     stem_loop       1508..1527
                     /label=T7 TE
     misc_feature    1534..1534
                     /label=stop
     polyA_site      1528..1541
                     /label=polya
     misc_feature    1501..1541
                     /label=BBa_B0012
     terminator      1501..1541
                     /label=BBa_B0012
     misc_feature    702..1541
                     /label=BBa_P0440
     misc_feature    659..1541
                     /label=BBa_S03706
     misc_feature    522..1541
                     /label=BBa_S03745
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aattcactga gtcatatgat cgatttgggt attaaagagg agaaacaatt gatgtgccaa
      121 tcgcgggggg ttttcgttca ggactacaac tgccacacac caccaaagct aactgacagg
      181 agaatccaga tggatgcaca aacacgccgc cgcgaacgtc gcgcagagaa acaggctcaa
      241 tggaaagcag caaatcccct gttggttggg gtaagcgcaa aaccagttaa ccgccctatt
      301 ctctcgctga atcgcaaacc gaaatcacga gtagaaagcg cactaaatcc gatagacctt
      361 acagtgctgg ctgaatacca caaacagatt gaaagcaacc tgcaacgtat tgagcgcaag
      421 aatcagcgca catggtacag caagcctggc gaacgcggca taacatgcag tggacgccag
      481 aaaattaagg gaaaatcgat tcctcttatc tagtactaga gccaggcatc aaataaaacg
      541 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      601 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
      661 gacggctagc tcagtcctag gtacagtgct agctactaga gaaagaggag aaatactaga
      721 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      781 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      841 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      901 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      961 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
     1021 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
     1081 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
     1141 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
     1201 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     1261 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     1321 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1381 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
     1441 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1501 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//