##gff-version 3
##sequence-region BBa_S03750 1 1859
BBa_S03750	.	engineered_region	840	1859	.	+	0	ID=BBa_S03745;Name=BBa_S03745
BBa_S03750	.	terminator	840	968	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03745
BBa_S03750	.	terminator	928	968	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03750	.	stem_loop	935	954	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03750	.	polyA_site	955	968	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03750	.	stop_codon	961	961	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03750	.	engineered_region	928	968	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03750	.	terminator	840	919	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03750	.	stem_loop	851	894	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03750	.	engineered_region	840	919	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03750	.	engineered_region	977	1859	.	+	0	ID=BBa_S03706;Name=BBa_S03706;Parent=BBa_S03745
BBa_S03750	.	promoter	977	1011	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_S03706
BBa_S03750	.	engineered_region	1020	1859	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03706
BBa_S03750	.	terminator	1731	1810	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S03750	.	stem_loop	1742	1785	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03750	.	engineered_region	1731	1810	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03750	.	ribosome_entry_site	1020	1031	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S03750	.	conserved_region	1024	1027	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03750	.	CDS	1038	1697	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S03750	.	tag	1658	1691	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S03750	.	engineered_tag	1698	1722	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S03750	.	CDS	1041	1657	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S03750	.	terminator	1819	1859	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S03750	.	stem_loop	1826	1845	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03750	.	polyA_site	1846	1859	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03750	.	stop_codon	1852	1852	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03750	.	engineered_region	1819	1859	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03750	.	engineered_region	1	831	.	+	0	ID=BBa_I759006;Name=BBa_I759006
>BBa_S03750
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatga
tcgatttgggtattaaagaggagaaacaattgatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgt
tgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaactt
acccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttc
aatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacagga
aagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaat
agaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcac
acaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatgg
aagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac
ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctg
ctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagagga
gaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaa
ggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcggg
ctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggca
agattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggt
acacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagaga
atgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa
agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggt
gcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccg
ctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata