LOCUS BBa_S03762 1583 bp DNA linear 25-Nov-2024 DEFINITION T7Promoter-RBS-RFP1-HixC-GFP2-RBS-RFP1-HixC-GFP2 (pSB1A2) ACCESSION BBa_S03762 VERSION BBa_S03762.1 FEATURES Location/Qualifiers misc_feature 18..18 /label=Transcription Start Site misc_feature 1..11 /label=Polymerase Binding Region misc_feature 12..23 /label=Initiation Region promoter 1..23 /label=BBa_R0085 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 misc_feature 50..52 /label=start CDS 50..511 /label=Coding sequence CDS 50..511 /label=BBa_I715022 misc_feature 520..545 /label=BBa_J44000 misc_feature 554..554 /label=extra base to maintain reading frame CDS 554..797 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 798..803 /label=Stop Codons (two in tandem) misc_feature 554..803 /label=BBa_I715020 misc_feature 816..819 /label=conserved RBS 812..823 /label=BBa_B0034 misc_feature 830..832 /label=start CDS 830..1291 /label=Coding sequence CDS 830..1291 /label=BBa_I715022 misc_feature 1300..1325 /label=BBa_J44000 misc_feature 1334..1334 /label=extra base to maintain reading frame CDS 1334..1577 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 1578..1583 /label=Stop Codons (two in tandem) misc_feature 1334..1583 /label=BBa_I715020 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc 61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt 541 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 781 tggatgaact atacaaataa taatactaga gaaagaggag aaatactaga tggcttcctc 841 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 901 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 961 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 1021 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 1081 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 1141 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 1201 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 1261 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt 1321 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 1381 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 1441 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 1501 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 1561 tggatgaact atacaaataa taa //