LOCUS       BBa_S03764               922 bp    DNA     linear       25-Nov-2024
DEFINITION  T.T.-HixC-RFP2-RBS-GFP1-HixC
ACCESSION   BBa_S03764
VERSION     BBa_S03764.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..163
                     /label=BBa_J44000
     misc_feature    172..172
                     /label=extra base (avoid frameshift)
     CDS             173..385
                     /label=Continue coding sequence
     misc_feature    386..390
                     /label=(two in tandem)
     CDS             172..391
                     /label=BBa_I715023
     misc_feature    404..407
                     /label=conserved
     RBS             400..411
                     /label=BBa_B0034
     misc_feature    418..420
                     /label=Start Codon
     CDS             418..888
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    418..888
                     /label=BBa_I715019
     misc_feature    897..922
                     /label=BBa_J44000
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtta tcaaaaacca tggtttttga taatactaga gtggtgctct
      181 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
      241 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
      301 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
      361 tgaaggtcgt cactccaccg gtgcttaata atactagaga aagaggagaa atactagatg
      421 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      481 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      541 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      601 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      661 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      721 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      781 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      841 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaata ctagagttat
      901 caaaaaccat ggtttttgat aa
//