LOCUS       BBa_S03765              1772 bp    DNA     linear       11-Dec-2025
DEFINITION  J31000:J31006
ACCESSION   BBa_S03765
VERSION     BBa_S03765.1  
FEATURES             Location/Qualifiers
     misc_feature    70..75
                     /label=Former SpeI site
     misc_feature    72..72
                     /label=Mutation to delete the SpeI site
     CDS             1..573
                     /label=Hin sequence
     primer_bind     1..3
                     /label=Fwd primer J31001
     primer_bind     551..570
                     /label=Rev primer J31001
     CDS             1..573
                     /label=BBa_J31000
     CDS             582..1772
                     /label=tetA(C) backwards
     misc_feature    582..584
                     /label=stop
     primer_bind     582..601
                     /label=Rev primer J31006
     primer_bind     1749..1772
                     /label=Fwd primer J31006
     CDS             582..1772
                     /label=BBa_J31006
ORIGIN
        1 atggctacta ttgggtatat tcgggtgtca acaattgacc aaaatatcga tttacagcgt
       61 aatgcgctta ccagtgcaaa ttgtgaccgc atttttgaag accgtatcag tggcaagatt
      121 gcaaaccgcc ccggcctgaa acgggcgtta aagtatgtaa ataaaggcga tactcttgtc
      181 gtctggaaat tagacagact gggccgtagc gtgaaaaatc tggtggcgtt aatatcagaa
      241 ttacatgaac gtggagctca cttccattct ttaaccgata gtattgatac cagtagcgcg
      301 atggggcgat tcttttttca tgtaatgtca gcactggccg agatggagcg agaattaatc
      361 gtcgagcgaa cccttgccgg actggctgcc gccagagcgc aaggacgact gggagggcgc
      421 cctcgggcga tcaacaaaca tgaacaggaa cagattagtc ggctattaga gaaaggccat
      481 cctcggcagc aattagctat tatttttggt attggcgtat ccaccttata cagatacttt
      541 ccggcaagca gtataaaaaa acgaatgaat taatactaga gttaggtcga ggtggcccgg
      601 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
      661 catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt
      721 ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
      781 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
      841 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
      901 tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
      961 gtggcggggc cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     1021 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     1081 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
     1141 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     1201 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     1261 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     1321 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     1381 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     1441 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tccacaggac gggtgtggtc
     1501 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
     1561 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat
     1621 agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag
     1681 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     1741 aggatgacga tgagcgcatt gttagatttc at
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