BBa_J44000
1
hixC
hixC binding site for Salmonella typhimurium Hin recombinase
2006-06-05T11:00:00Z
2015-08-31T04:08:48Z
Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase
[http://www.genetics.org/cgi/content/abstract/149/4/1649]
A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
false
true
_71_
0
606
61
In stock
true
Standard BioBrick prefix and suffix were added to the 26 bp sequence.
true
Missouri Western and Davidson Groups, Todd Eckdahl
BBa_I715020
1
GFP2
Carboxyl Half of GFP (aka GFP2)
2007-06-21T11:00:00Z
2015-08-31T04:07:49Z
A
Released HQ 2013
A
false
false
_120_
0
1491
9
In stock
false
A
true
Will DeLoache
annotation1935360
1
extra base to maintain reading frame
range1935360
1
1
1
annotation1935362
1
Stop Codons (two in tandem)
range1935362
1
245
250
annotation1935361
1
Coding Carboxyl half of GFP (aka GFP2)
range1935361
1
1
244
BBa_I715019
1
GFP1
Amino Half of GFP (aka GFP1)
2007-06-21T11:00:00Z
2015-08-31T04:07:49Z
A
Released HQ 2013
A
false
false
_120_
0
1491
9
In stock
false
A
true
Will DeLoache
annotation1935356
1
Start Codon
range1935356
1
1
3
annotation1935357
1
Coding Amino Portion GFP (aka GFP1)
range1935357
1
1
471
BBa_S03770
1
BBa_S03770
RBS-GFP1-hixC-GFP2
2007-08-01T11:00:00Z
2015-05-08T01:14:30Z
false
true
_120_
0
1491
9
It's complicated
false
false
Will DeLoache
component1940669
1
BBa_I715020
component1940662
1
BBa_B0034
component1940666
1
BBa_J44000
component1940665
1
BBa_I715019
annotation1940666
1
BBa_J44000
range1940666
1
498
523
annotation1940665
1
BBa_I715019
range1940665
1
19
489
annotation1940669
1
BBa_I715020
range1940669
1
532
781
annotation1940662
1
BBa_B0034
range1940662
1
1
12
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I715019_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J44000_sequence
1
ttatcaaaaaccatggtttttgataa
BBa_S03770_sequence
1
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaatactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_I715020_sequence
1
taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z