LOCUS       BBa_S03777               421 bp    DNA     linear       23-Dec-2024
DEFINITION  hixC-GFP2-TT
ACCESSION   BBa_S03777
VERSION     BBa_S03777.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     misc_feature    35..35
                     /label=extra base to maintain reading frame
     CDS             35..278
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    279..284
                     /label=Stop Codons (two in tandem)
     misc_feature    35..284
                     /label=BBa_I715020
     stem_loop       304..347
                     /label=stem_loop
     misc_feature    293..372
                     /label=BBa_B0010
     terminator      293..372
                     /label=BBa_B0010
     stem_loop       388..407
                     /label=T7 TE
     misc_feature    414..414
                     /label=stop
     polyA_site      408..421
                     /label=polya
     misc_feature    381..421
                     /label=BBa_B0012
     terminator      381..421
                     /label=BBa_B0012
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagtaagaa tggaatcaaa gttaacttca
       61 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      121 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      181 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      241 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
      301 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      361 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      421 a
//